Abstract
Neurospora crassa Em 5297a can utilize sodium Β-glycerophosphate as a sole phosphorous source (in the place of KH2PO4). Under these conditions a repressible alkaline phosphatase is elaborated which has different pH optimum towards Β-glycerophosphate (10.2) and pyrophosphate (9.0) as substrates. This enzyme does not require any metal ion for its activity and could be assayed in the presence of EDTA. However, under conditions of cobalt toxicity, the activity of this enzyme is high and is decreased in copper and nickel toxicities.
Similar content being viewed by others
References
Fiske, C. H. and Subba Rao, Y. (1925)J. Biol. Chem.,66, 375.
Grove, G. and Murzluf, G. (1980)J. Bacterol.,141, 1470.
Hochberg, M. L. and Sargent, M. L. (1973)Can J Microbiol.,19, 1487.
Jacobs, M. M., Nyc, J. F. and Brown, D. M. (1971)J. Biol.Chem.,246, 1419
Kadner, R. J., Nyc J. F. and Brown, D. M. (1968)Biol Chem.,243, 3076.
Kuo, M. H. and Blumenthal, H. J. (1961)Nature (London),190, 29.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol Chem.,193, 265
Nyc, J. F., Kadner, R. J. and Crocken, B. J. (1966)J. Biol. Chem.,241, 1468.
Nyc, J. F. (1967)Biochem. Res. Commun.,27, 183.
Venkateswerlu, G. and Sivarama Sastry, K. (1973)Biochem. J. 132, 673.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Venkateswerlu, G., Sivarama Sastry, K. A new repressible alkaline phosphatase inNeurospora crassa EM 5297a. J. Biosci. 4, 275–279 (1982). https://doi.org/10.1007/BF02702739
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02702739