Abstract
The major protein from glanded cottonseed has been isolated in a homogeneous form. Its S20 w value at 1 protein concentration is 6S in 1 M NaCl solution. It contains 1 carbohydrate and is free from phosphorus, gossypol (bound or free) and nucleic acid impurities. It consists of atleast seven non-identical subunits. The protein has an ultraviolet absorption maximum at 278 nm and fluorescence excitation and emission maxima at 280 nm and 325 nm respectively. Optical rotatory dispersion and circular dichroism measurements indicate that the protein consists predominantly of Β-structure and random coil. The observed near-ultraviolet circular dichroic bands can be attributed to tyrosine, phenylalanine and tryptophan residues of the protein.
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Abbreviations
- TEMED:
-
N N N’ N’-tetramethylethylene diamine
- ORD:
-
optical rotatory dispersion
- CD:
-
circular dichroism
- SDS:
-
sodium dodecyl sulfate
- DEAE cellulose:
-
diethyl aminoethyl cellulose
- PAGE:
-
polyacrylamide gel electrophoresis
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Mohan Reddy, I., Appu Rao, A.G. & Narasinga Rao, M.S. Isolation and characterization of the 7S protein from glanded cottonseed (Gossypium herbacium). J Biosci 4, 197–208 (1982). https://doi.org/10.1007/BF02702730
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DOI: https://doi.org/10.1007/BF02702730