Abstract
The acidic α-mannosidase was purified 4400-fold by affinity chromatography on Concavalin A-Sepharose and heat treatment at 65‡C in the presence of 1 mM zinc ion. The enzyme did not resolve into multiple forms as in the case of enzymes from human liver and human kidney. The pH optimum of the enzyme was 4.2 in citrate-phosphate buffer. The Km value for p-nitrophenyl-α-D-mannose was 1.9 mM. The molecular weight of the enzyme determined by gel filtration was 300,000. The enzyme contained 10.6% neutral sugars.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andrews, P. (1964)Biochem. J.,91, 222.
Bishayee, S. and Bachhawat, B. K. (1974)Neurobiology,4, 48.
Bossmann, H. B. (1972)Biochim, Biophys. Acta,258, 265.
Carroll, M., Dance, N., Masson, P. K., Robinson, D., and Winchester, B. G. (1972)Biochem. Biophys, Res. Commun.,49, 579.
Davis, B. J. (1964)Ann. N. Y. Acad. Sci.,121, 404.
Dewald, B. and Touster, O. (1973)J. Biol. Chem.,248, 7223.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. (1956)Anal. Chem.,28, 350.
Forsee, W. T. and Schutzbach, J. S. (1981)J. Biol. Chem.,256, 6577.
Hocking, J. D., Jolly, R. D. and Batt, R. D. (1972)Biochem., J.,128, 69.
Kjellman, B., Gamstrop, I., Brun, A., Ockermann, P. A. and Palmgren, B. (1969)J. Pediat.,76, 366.
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. (1951)J. Biol. Chem.,193, 265.
Marinkovic, D. V. and Marinkovic, J. N. (1976)Biochem. J.,155, 217.
Ockermann, P. A. (1967)Lancet,2, 239.
Phillips, N. C., Robinson, D. and Winchester, B. G. (1976)Biochem. J.,153, 579.
Shoup, V. A. and Touster, O. (1976)J. Biol. Chem.,251, 3845.
Snaith, S. M. and Levvy, G. A. (1968)Nature,218, 91.
Snaith, S. M. and Levvy, G. A. (1973)Adv. Carbhyd. Chem. Biochem.,28, 401.
Snaith, S. M. (1975)Biochem. J.,147, 83.
Surolia, A., Prakash, N., Bishyaee,S.and Bachhawat, B.K.(1973)Indian J. Biochem. Biophys.,10, 145.
Suzuki, I., Kushida, H. and Shida, H. (1969)J. Biochem. (Tokyo),41, 334.
Tulsiani, D. R. P., Opheim, D. J. and Touster, O. (1977)J. Biol. Chem.,252, 3227.
Weber, K. and Osborn, M. (1969)J. Biol. Chem.,244, 4406.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Khan, F.A., Basu, D. Isolation and characterization of lysosomal alpha-mannosidase of placental tissue. J Biosci 4, 133–138 (1982). https://doi.org/10.1007/BF02702721
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02702721