Abstract
A novel polycationic ionen was synthesized and fractionated on carboxymethyl-Sephadex using a salt gradient in 7M urea. A series of oligomers of discrete length were characterised by ultraviolet spectra. The ultraviolet spectra of oligomers revealed a new band centred at 232.5 nm which was probably due to exciton splitting. Thermal denaturation studies indicated both stabilization of the helix conformation and a higher degree of cooperativity in the melting of DNA (oligomers)n complex as compared to native calf thymus DNA. Ionen oligomers exhibited large extrinsic Cotton effect at 232.5 nm which could be attributed to exciton interaction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- uv:
-
ultraviolet
- CD:
-
circular dichroism
References
Bhat, G., Roth, A. C. and Day, R. A. (1977),Biopolymers,16, 1713.
Davydov, A. S. (1962)Theory of molecular excitons (Translated by M. Kasha and M. Oppenheimer) (New York: McGraw Hill Book Company).
Day, R. A., Bhat, G., Lin, L. C., Roth, A. C. Mulimani, V. H. and Krueger, R. C. (1978)Biochem. Biophys. Res. Communi.,84, 4, 969.
Day, R. A., Roth, A. C., Bhat, G. and Mulimani, V. H. (1978),Feb. Proc.,37, 1670.
Gabbay, E. J. (1978)Bio-organic Chemistry,111, 33.
Harada, S, L, Chen, K., Nakanishi (1975)J. Am. Chem. Soc.,97, 5345.
Ivanov, V. I., Minchenkova, L. H., Schyalkins, A. K. and Palotayev, A. L. (1973),Biopolymers,12, 80.
Kasha, M. (1963)Radiation Research,20, 55.
Kawashima, S., Toshio, A. (1978)J. Biochem.,84, 343.
Kawashima, S., Inoue, S. and Ande, T. (1969)Biochem. Biophys., Acta,186, 145.
Levinson. G. L., Simpson, W. T., Curtis, W. J. (1957)J. Am. Chem. Soc.,79, 4314.
Moffitt, M. (1956)Proc. Natl. Acad. Sci.,42, 736.
McRae, A. G. and Kasha, M..(1958)J. Chem. Phys.,28, 721.
Mulimani, V. H., Bhat, G. and Day, R. A. (1977), Abstracts Biology 168, Abstract 17, 175th ACS National meeting Chicago, USA.
Mita, K., Zama, M. and Ichimura, S. (1977)Biopolymers,16, 1993.
Mulimani, V. H. and Day, R. A. (1981)Indian J. Biochem. Biophys.,18, 157.
Mulimani, V. H and Day, R. A. (1981)Indian J. Biochem. Biophys., (In press).
Mulimani, V. H. and Day, R. A. (1980) Second Congress of the Federation of Asian and Oceanian Biochemists and Golden Jubilee of Annual Meeting of Society of Biological Chemists, India Indian Institute of Science, Bangalore, December 14–18, p. 98.
Mulimani, V. H. and Day, R. A. (1981) Modern Aspects of Bio-inorganic Chemistry sponsored by University Grants Commission, New Delhi, Jadavpur University, Department of Chemistry, Calcutta, Jan. 31 to Feb. 3, p. 43.
Rembaum, A., Singer, S. and Keyzer, H. (1967)Polymer Letters,7, 395.
Rembaum, A., Baugartner, W. and Eisenberg, A. (1968)J. Polymer Sci. B,6, 59
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mulimani, V.H., Roth, A.C. & Day, R.A. Interaction of aromatic ionen oligomers with DNA. J Biosci 4, 127–132 (1982). https://doi.org/10.1007/BF02702720
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02702720