Abstract
An endogenous inhibitor of calcium activated neutral proteinase has been purified from human placenta. The procedure included chromatography on DEAE cellulose, Ultrogel AcA 22 and milli calcium activated neutral proteinase-sepharose in succession. Endogenous calcium activated neutral proteinase inhibitor was a tetramer with identical subunits of molecular weight 68 kDa. It was specific for milli calcium activated neutral proteinase (Calpain II) which is inhibited by the formation of an inactive enzyme-inhibitor complex and not by sequestering Ca2+ from the medium. Although micro calcium activated neutral proteinase (Calpain I) was not inhibited by endogenous calcium activated neutral proteinase inhibitor, it was protected from autolysis in the presence of the inhibitor. The placental endogenous calcium activated neutral proteinase inhibitor thus regulates Ca2+ activated proteolysis by ensuring micro calcium activated neutral proteinase activity, while inhibiting milli calcium activated neutral proteinase.
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Abbreviations
- CANP:
-
Calcium activated neutral proteinase
- ECI:
-
endogenous CANP inhibitor
- SDS:
-
sodium dodecyl sulphate
- PAGE:
-
Polyacrylamide gel electrophoresis
- PBS:
-
phosphate buffer saline
- M r :
-
molecular weight
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Shastri, R., Shailaja, B. & Anandaraj, M.P.J.S. Endogenous inhibitor of calcium activated neutral proteinase from human placenta: Purification and possible mechanism of proteinase regulation. J Biosci 15, 435–442 (1990). https://doi.org/10.1007/BF02702685
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DOI: https://doi.org/10.1007/BF02702685