Magnetic resonance studies of dynamic organisation of lipids in chloroplast membranes

Abstract

Spinach chloroplast membranes and aqueous dispersions of their extracted lipids have been studied by spin label (stearic acid) electron spin resonance and carbon-13 nuclear magnetic resonance techniques. Combined with electron microscope studies, first systematic evidence is found for the existence of a dynamic lipid-bilayer structure in the chloroplast membranes.

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Abbreviations

MGDG:

Monogalactosyl diacylglycerol

DGDG:

digalactosyl diacylglycerol

ESR:

electron spin resonance

13C-NMR:

carbon-13 nuclear magnetic resonance

TMS:

tetramethylsilane

References

  1. Anderson, J. M. (3975)Biochim. Biophys. Acta,416, 191.

    Google Scholar 

  2. Arnon, D. I., Allen, M. B. and Whutley, F. R. (1956)Biochim. Biophys. Acta,20, 449.

    PubMed  Article  CAS  Google Scholar 

  3. Bangham, A. D. (1968)Prog. Biophys. Mol. Biol.,18, 29.

    Article  CAS  Google Scholar 

  4. Cannon, B., Polnaszek, C. F., Butler, K. W., Ericksson, L. E. G. and Smith, I. C. P. (1975)Arch. Biochem. Biophys.,167, 505.

    PubMed  Article  CAS  Google Scholar 

  5. Curatolo, W. (1987)Biochim. Biophys. Acta,906, 137.

    PubMed  CAS  Google Scholar 

  6. Kates, M., Paoletti, R. and Kritchevsky, D. (1970)Adv. Lipid. Res.,8, 225.

    CAS  Google Scholar 

  7. Larsson, K. and Puang-Ngern, S. (1979) inAdvances lipid research (eds L.A. Appelquist and C. Liljenberg) (Amsterdam: Elsvier/North Holland Biomedical Press) p. 27.

    Google Scholar 

  8. Lucy, J. A. and Glauert, A. M. (1964)J. Mol. Biol.,8, 727.

    PubMed  CAS  Article  Google Scholar 

  9. McConnell, H. M. and McFarland, B. G. (1972)Ann. N. Y. Acad. Sci.,195, 207.

    PubMed  Article  CAS  Google Scholar 

  10. Mühlethaler, K., Moor, H. and Szarkowski, J. W. (1965)Planta,67, 305.

    Article  Google Scholar 

  11. Murphy, D. J. (1986)Biochim. Biophys. Acta,864, 33.

    CAS  Google Scholar 

  12. Nichols, B. W. (1963)Biochim. Biophys. Acta,70, 417.

    PubMed  Article  CAS  Google Scholar 

  13. Quin, P. J. and Williams, W. P. (1983)Biochim. Biophys. Acta,737, 223.

    Google Scholar 

  14. Rottem, S., Hubbel, W. L., Hayiluk, L. and McConnell, M. M. (1970)Biochim. Biophys. Acta,219, 104.

    PubMed  Article  CAS  Google Scholar 

  15. Seelig, J. and Hasselbach, W. (1971)Eur. J. Biochem.,21, 17.

    PubMed  Article  CAS  Google Scholar 

  16. Sternin, E., Fine, B., Bloom, M., Tilock, C. P. S., Wong, K. F. and Cullis, P. R. (1988)Biophys. J.,54, 689.

    PubMed  CAS  Google Scholar 

  17. Van Gurp, M., Van Ginkel, G., Levine, Y. K. (1988)Biochim. Biophys. Acta,938, 71.

    Article  Google Scholar 

  18. Vernon, L. P. (1960)Anal. Chem.,32, 1144.

    Article  CAS  Google Scholar 

  19. Vignais, P. M., Devaux, P. and Colbeau, A. (1975) inBiomembranes-lipids, proteins and receptors (eds R. M. Burton and L. Packer) (Missouri: BI Science Publications) p. 318.

    Google Scholar 

  20. Weier, T. E. and Benson, AAA. (1966) inBiochemistry of chloroplasts (ed. T. W. Goodwin) (New York, London: Academic Press) vol. 1, p, 91.

    Google Scholar 

  21. YashRoy, R. C. (1980)Fluidity and lipid protein interactions in the chloroplast membranes: A. magnetic resonance study; Ph. D. thesis, Carleton University, Ottawa, Canada

    Google Scholar 

  22. YashRoy, R. C. (1987a)Indian J. Biochem. Biophys.,24, 177.

    PubMed  CAS  Google Scholar 

  23. YashRoy, R. C. (1987b)J. Biochem. Biophys. Methods,15, 229,

    PubMed  Article  CAS  Google Scholar 

  24. YashRoy, R. C. (1990a)J. Biosci.,15, 93.

    CAS  Google Scholar 

  25. YashRoy, R. C. (1990b)J. Biochem. Biophys. Methods,20, 353.

    PubMed  Article  CAS  Google Scholar 

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Yashroy, R.C. Magnetic resonance studies of dynamic organisation of lipids in chloroplast membranes. J Biosci 15, 281–288 (1990). https://doi.org/10.1007/BF02702669

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Keywords

  • Chloroplast
  • membranes
  • lipids
  • nuclear magnetic resonance
  • electron spin resonance