Abstracts
The effect of sodium dodecyl sulphate, urea, guanidinium hydrochloride and heat on the oligomeric structure of the 11 S protein of sunflower has been determined. Sodium dodecyl sulphate directly dissociates the protein to 2 S subunits, whereas urea and guanidinium hydrochloride dissociate it through an intermediate 7 S protein. Heating the protein at 90‡C for 20 min caused dissociation of the 11 S protein, without any precipitation.
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Abbreviations
- mol. wt.:
-
Molecular weight
- SDS:
-
sodium dodecyl sulphate
- GuHCl:
-
guanidinium hydrochloride
- PAGE:
-
polyacrylamide gel electrophoresis
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Rahma, E.H., Narasinga Rao, M.S. Effect of denaturants on the oligomeric structure of the 11 S protein of sunflower (Helianthus annum). J Biosci 3, 407–416 (1981). https://doi.org/10.1007/BF02702629
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DOI: https://doi.org/10.1007/BF02702629