Abstract
The total and ouabain-dependent rate constants of efflux of zinc (Zn) ions from lymphocytes isolated from healthy subjects were measured in vitro in an environment containing calcium (Ca) and magnesium (Mg) ions. Both the total (ERCt-Zn) and ouabain-dependent (ERCos-Zn) rate constants were higher in the presence of Mg2+, with the the oubain-dependent efflux significantly different 0.29±0.07 vs 0.13±0.02 with and without Mg2+, respectively (p<0.001). After the addition of verapamil, an increase of ERCE-Zn was observed in both ionic environments and was higher and statistically significant in the presence of Mg2+: 1.94±0.64 vs 2.97±1.16 (p<0.025). These results suggest that verapamil has an enhancing effect on Zn efflux from isolated lymphocytes, suggesting that calcium channel blockers might result in better Zn homeostatic regulation in diseases of the cardiovascular system.
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References
T. J. B. Simons, Intracellular free Zn and Zn buffering in human red blood cells,J. Membr. Biol. 123, 63–71 (1991).
T. J. B. Simson, Calcium-dependent Zn efflux in human red blood cells,J. Membr. Biol. 123, 73–81 (1991).
D. J. Bobilya, M. Briske-Anderson, and P. G. Reeves, Zn transport into endothelial cell is a facilitated process,J. Cell. Physiol. 151, 1–7 (1992).
R. D. Raffaniello, S.-Y. Lee, S. Teichberg, and R. A. Wapnir, Distinct mechanisms of zinc uptake at the apical and basolateral membranes of caco-2 cells,J. Cell. Physiol. 152, 356–361 (1992).
E Tacnet, E Lauthier, and P. Ripoche, Mechanisms of zincy transport into pig small intestine brush-border membrane vesicles,J. Physiol. 465, 57–72 (1993).
S. Ripa and R. Ripa, Zinc cellular traffic: physiopathological considerations,Minerva Med. 86, 37–43 (1995).
J. G. Henrotte, M. Santarromana, G. Franck, P. Guicheney, R. Boulu, and R. Bourdon, High cardiac zinc level in spontaneously hypertensive rats,J. Hypertens. 10, 553–559 (1992).
A. Boyum, Isolation of mononuclear cells and granulocytes from blood. II. Isolation of mononuclear cells by centrifugation and of granulocytes by combining centrifugation and sendimentation of 1 g,Scand. J. Clin. Lab. Invest. 21(Suppl. 97), 77–85 (1968).
A. Noworolska, Antygeny onkopłodowe w komórkach szeregu mielocytarnego, Praca doktorska. AM Wrocław (1984).
A. M. Heagerty, R. F. Bing, M. Miluer, H. Thuston, and J. W. Shales, Leucocyte membrane sodium transport in normotensive populations; dissociation of abnormalities of sodium efflux from raised blood pressure,Lancet 2, 894–905 (1982).
J. Durlach,Magnesium in Clinical Practice, PZWL, Warsaw, pp. 24–25 (1991).
M. P. Blaustein, J. Zhang, L. Chen, and B. P. Hamilton, How does salt retention blood pressure?Am. J. Physiol. Regul. Integr. Comp. Physiol. 290, R514-R523 (2006).
P. Manunta, M. Ferrandi, E. Messaggio, and P. Ferrari, A new antihypertensive agent that antagonizes the prohypertensive effect of endogenous ouabain and adducin,Cardiovasc. Hematol. Agents Med. Chem. 4, 61–66 (2006).
W. Schoner and G. Scheiner-Bobis, Endogenous cardiac glycosides: hormones using the sodium pump as signal transducer,Semin. Nephrol. 25, 343–351 (2005).
W. Schoner, N. Bauer, J. Muller-Ehmsen, et al., Ouabain as a mammalian hormone,Ann. NY Acad. Sci. 986, 678–684 (2003).
L. V. Rossoni, L. Dos Santos, L. A. Barker, and D. V. Vassallo, Ouabaine changes arterial blood pressure and vascular reactivity to phenylephrine in L-NAME-induced hypertension,J. Cardiovasc. Pharmacol. 41, 105–116 (2003).
H. Dahlheim, C.L. White, and J. Rothemund, Effect of zinc depletion on angiotensin I-converting enzyme in arterial walls and plasma of the rat,Miner. Electrolyte Metab,15, 125–131 (1989).
S. Tubek, Selected zinc metabolism parameters in relation to insulin and renin - angiotensin - aldosterone system and blood pressure in healthy subjects: sex differences,Biol. Trace Element Res. 114, 65–72 (2006).
B. S. Huang, M. Kudlac, R. Kumarathasan, and F. H. Leenen, Digoxin prevents ouabain and high salt intake-induced hypertension in rats with sinoaortic denervation,Hypertension 34, 733–738 (1999).
F. Atlihan, T. Soylemezoglu, A. Gokce, G. Guvendik, and O. Satici, Zinc and copper in congestive heart failure,Turk. J. Pediatr. 32, 33–38 (1990) (abstract).
C. Pieri, R. Recchioni, F. Moroni, et al., Ligand and voltage gated sodium channels may regulate electrogenic pump activity in human, mouse, and rat lymphocytes,Biochem. Biophys. Res. Commun. 160, 999–1002 (1989).
J. Cortijo, J. V. Esplugues, and B. Sarria, Zinc as a calcium antagonist; a pharmacological approach in strips of rat aorta,IRCS Med. Sci. Cancer 13, 292–293 (1985).
J. T. Rogers and C. M. Wood, Characterization of branchial lead-calcium interaction in the freshwater rainbow trout,Oncorhynchus mykiss, J. Exp. Biol. 207, 813–825 (2004).
S. Tubek, Effect of aldosterone receptor blockade by spironolactone on zinc efflux rate constants from lymphocytes of patients with arterial hypertension,Post. Med. Klin. Dośw 3, 27–33 (1994).
C. Dacquet, G. Loirand, C. Mironneau, J. Mironneau, and R Pacaud, Spironolactone inhibition of contraction and calcium channels in rat portal vein,Br. J. Pharmacol. 92, 535–544 (1987).
J. Mironneau, I. Sayet, L. Rakotoarisoa, C. Dacquet, and C. Mironneau, Interactions of spironolactone with (+)-[3H]-isradipine and (-)-[3H]-desmethoxyverapamil binding sites in vascular smooth muscle,Br. J. Pharmacol. 101, 6–7 (1990).
H. J. Kramer, K. Glanzer, and M. Sorger, The role of endogenous inhibition of Na-K-ATPase in human hypertension: sodium pump activity as a determinant of peripheral vascular resistance,Clin. Exp. Hypertens. A 7, 769–782 (1985).
J. G. Henrotte, M. Santarromana, G. Franck, and R. Bourdon, Blood and tissue zinc levels in spontaneously hypertensive rats,J. Am. Coll. Nutr. 9, 340–344 (1990).
E. I. Astashkin, M. G. Glezer, A. B. Khodorova, G. G. Arabidze, L. L. Orlov, and O. B. Poliakova, Calcium 2+ response of lymphocytes to standard test-substances in patients with ischemic heart disease and heart failure,Kardiologiia 41, 67–69 (2001).
B. Hennig, Y. Wang, S. Ramasamy, and C. J. McClain, Zinc deficiency alters barrier function of cultured porcine endothelial cells,J. Nutr. 122, 1242–1247 (1992).
B. Hennig, Y. Wang, S. Ramasamy, and C. J. McClain, Zinc protects against tumor necrosis factor-induced disruption of porcine endothelial cell monolayer inegrity,J. Nutr. 123, 1003–1009 (1993).
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Tubek, S. Zinc ions efflux from lymphocytes in vitro in the presence of a calcium and magnesium ionic environment and its changes following administration of verapamil. Biol Trace Elem Res 117, 15–21 (2007). https://doi.org/10.1007/BF02698080
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DOI: https://doi.org/10.1007/BF02698080