Abstract
The classical Ogston-Laurent gel model of size exclusion chromalography was modified to include both dextran fibers and cross-links. The dimensions of Sephadex gel fibers were determined from the experimental data in the literature and the microstructural study o’ Sephadex gel. The corresponding radii of the dextran chain and the cross-linking were calculated to be 7.8 x l(10*-8and 4.8 x 10*-8 cm, respectively. The lengths of the dextran chain varies from 1.45 x 1012 (G-200) lo 9.46 x 1012 cm/m/(G-25) while the length of the cross-link varies from 0.14 x 1012 (G-200) to 3.18 x 1012 cm/m/ (G-25). The partial specific volume of swollen Sephadex gel material was calculated to be 0.586 m//g.
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Koo, YM. Estimation of fiber radii and lengths of sephadex gel by the modified Ogston model. Korean J. Chem. Eng. 6, 254–258 (1989). https://doi.org/10.1007/BF02697688
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DOI: https://doi.org/10.1007/BF02697688