The in vitro immunomodulatory effects of glyconutrients on peripheral blood mononuclear cells of patients with chronic fatigue syndrome

  • Darryl M. See
  • Paul Cimoch
  • Shioweh Chou
  • Jennifer Chang
  • Jeremiah Tilles


In humans, eight monosaccharides are required for the synthesis of glycoproteins. Dietary supplements that supply these crucial sugars are known as glyconutrients. A glyconutrient compound was added to Peripheral Blood Mononuclear Cells (PBMC) isolated from normal controls and patients with the Chronic Fatigue Syndrome (CFS), a disease associated with immune dysregulation. The in vitro immunomodulatory effects were investigated. Cell surface expression of the glycoproteins CD5, CD8, and CD11a were significantly lower in patients with CFS compared to normal controls. Addition of glyconutrient homogenate to PBMC from patients with CFS stimulated with phytohemagglutinin significantly increased the expression of each glycoprotein. Furthermore, natural killer (NK) cell function was reduced in CFS patients. The glyconutrient preparation significantly enhanced NK cell activity versus human herpes virus 6 (HHV-6)-infected H9 cells in an 8 h51Cr release assay compared to placebo for PBMC from patients with CFS (p<.01). Finally, apoptosis was significantly higher in patients with CSF. The percentage of apoptotic cells was significantly decreased in PBMC from patients with CFS that had been incubated for 48 h with glyconutrients. Thus, glyconutrients improved abnormal immune parameters in vitro in patients with CFS.


Natural Killer Peripheral Blood Mononuclear Cell Chronic Fatigue Syndrome Immunomodulatory Effect Cell Surface Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer 1998

Authors and Affiliations

  • Darryl M. See
    • 1
  • Paul Cimoch
    • 2
  • Shioweh Chou
    • 1
  • Jennifer Chang
    • 1
  • Jeremiah Tilles
    • 1
  1. 1.Department of MedicineUniversity of California, IrvineOrange
  2. 2.Center for Special ImmunologyIrvine

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