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Chromatographia

, Volume 24, Issue 1, pp 347–350 | Cite as

High-performance liquid chromatographic determination of major organic acids in apple juices and ciders

  • D. Blanco Gomis
  • M. J. Moran Gutierrez
  • M. D. Gutierrez Alvarez
  • A. Sanz Medel
Article

Summary

A reversed-phase high-pressure liquid chromatographic method is presented for the simultaneous separation and determination of malic, citric, lactic, succinic and ascorbic acids in apple juices and ciders. After filtration and/or degasification, the organic acids in the sample are separated on a LiChrosorb/C18 column and quantified by using a rapid diode array detector.

The method is considered to be a suitable choice for the accurate and precise determination (C.V. 5%) of these compounds.

Key Words

High-performance liquid chromatography Reversed-phase Organic acids Apple juice Cider 

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References

  1. [1]
    Association Francaise de Normalisation (1978), NFV76, 002–006.Google Scholar
  2. [2]
    D. A. Heatherbell, J. Sci. Food Agr.,25, 1095 (1974).CrossRefGoogle Scholar
  3. [3]
    D. W. Backer, J. Assoc. Offic. Anal. Chemist,56, 1257 (1973).Google Scholar
  4. [4]
    Boehringer, Mannheim (Germany), (1975/1976).Google Scholar
  5. [5]
    H. Rebelein, Dent. Lebensm. Rundschau,63, 327 (1967).Google Scholar
  6. [6]
    R. Schwarzenbach, J. Chromatogr.,251, 339 (1982).CrossRefGoogle Scholar
  7. [7]
    V. T. Turkelson, M. Richards, Anal. Chem.,50, 1420 (1978).CrossRefGoogle Scholar
  8. [8]
    A. G. H. Lee, S. J. Smith, Flüssiges Obst,52, 599 (1985).Google Scholar
  9. [9]
    R. Bushway, J. Bureau, D. McGann, J. Food Sci.,49, 75 (1984).Google Scholar
  10. [10]
    R. Andersson, B. Hedlung, Z. Lebensm. Unters. Forsch.,176, 440 (1983).CrossRefGoogle Scholar
  11. [11]
    R. H. Evans, A. W. van Soestberben, K. A. Ristow, J. Assoc. Off. Anal. Chem.,66 (6), 1517 (1983).Google Scholar
  12. [12]
    E. D. Coppola, Food Technol.,36 (4), 88 (1984).Google Scholar
  13. [13]
    H. J. Jeuring, A. Brands, P. van Doorninck, Z. Lebensm. Unters. Forsch.,168, 185 (1979).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • D. Blanco Gomis
    • 1
  • M. J. Moran Gutierrez
    • 1
  • M. D. Gutierrez Alvarez
    • 1
  • A. Sanz Medel
    • 1
  1. 1.Departamento de Quimica Fisica y Analitica, Facultad de QuimicaUniversidad de OviedoOviedoSpain

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