High-performance liquid chromatographic determination of major organic acids in apple juices and ciders
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A reversed-phase high-pressure liquid chromatographic method is presented for the simultaneous separation and determination of malic, citric, lactic, succinic and ascorbic acids in apple juices and ciders. After filtration and/or degasification, the organic acids in the sample are separated on a LiChrosorb/C18 column and quantified by using a rapid diode array detector.
The method is considered to be a suitable choice for the accurate and precise determination (C.V. 5%) of these compounds.
Key WordsHigh-performance liquid chromatography Reversed-phase Organic acids Apple juice Cider
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