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Study of the separation of lactose, lactulose and galactose by liquid chromatography using cationic ion-exchange resin columns

Chromatographia volume 41, pages 561–567 (1995)Cite this article

Summary

A strong acid cation-exchange resin, Dowex AG50W-X8 in potassium, calcium or sodium form, with water as eluent, has been used to separate three sugars (lactose, lactulose and galactose). The linearity of lactulose equilibrium isotherms was verified. Thermodynamic and kinetic parameters were thus determined by the moment method. Evaluation of the parameters revealed in this work can be considered as the first step in scaling up the process towards industrial use. On the other hand, the equilibrium stage model, which takes mass transfer resistance into account, is shown to provide a fair representation of the system’s behaviour.

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Abbreviations

u e :

interstitial velocity (ms“−1)

L:

column length (m)

ε:

interparticle porosity

Q :

mobile phase flow rate (m3s−1)

C n−1 :

mobile phase concentration in stage numbern−1 (gg−1)

Cn :

mobile phase concentration in stage numbern (gg−1)

Vm :

column hold-up volume (m3)

Vs :

total stationary phase volume (m3)

N :

total number of stages

k :

overall mass-transfer coefficient (s−1)

K :

distribution coefficient of each solute

DL :

axial dispersion coefficient (m2s−1)

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Authors and Affiliations

  1. Département Chimie, Laboratoire des Procédés de Séparation, U.A. INRA, IUT de Rennes, Rue du Clos Courtel, 35014, Rennes Cedex, France

    K. Dendene, L. Guihard, B. Balannec & B. Bariou

Authors
  1. K. Dendene
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  2. L. Guihard
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  3. B. Balannec
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  4. B. Bariou
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Dendene, K., Guihard, L., Balannec, B. et al. Study of the separation of lactose, lactulose and galactose by liquid chromatography using cationic ion-exchange resin columns. Chromatographia 41, 561–567 (1995). https://doi.org/10.1007/BF02688086

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  • DOI: https://doi.org/10.1007/BF02688086

Key Words

  • Column liquid chromatography
  • Cation ion-exchange resins
  • Lactose, lactulose and galactose