Summary
Trans fatty acids (TFA) in edible fats are of interest, since they are suspected to influence serum lipid levels. Thus, to estimate the atherogenic potency of edible fats a precise analytical method for the determination of TFA is required. The present study does not only aim at the quantitative analysis of total C18:1 TFA, but also at the determination of the isomeric pattern oftrans-octadecenoic acids in different fats.
The combination of argentation TLC with high resolution gas chromatography allows the quantification of 10trans- and 9cis-octadecenoic acid peaks. This method can be applied to hydrogenated vegetable fats as well as to ruminant fats and tissue lipids from different species. Based on this two-dimensional chromatography the intake of single TFA isomers with food can be determined, which is of importance with respect to the hypothesis, that the negative metabolic activity originates only from certain isomers, and to the considerable variation of isomeric patterns in different edible fats.
Based on the precise determination of the totaltrans-octadecenoic acid content in milk fats by TLC and GC, a formula has been derived by regression analysis, which allows the evaluation of total C18:1 TFA contents from a routine GC analysis. Though the 61 milk fats used covered a wide scope of feeding and lactation conditions, a correlation coefficient of 0.9991 was achieved.
Similar content being viewed by others
References
P. L. Zock, “Dietary fatty acids and risk factors for coronary heart disease”, thesis, Wageningen, 1995.
D. Precht, J. Molkentin, Kieler Milchwirtsch. Forschungsber.46, 249 (1994).
R. P. Mensink, M. B. Katan, N. Engl. J. Med.323, 439 (1990).
P. L. Zock, M. B. Katan, J. Lipid Res.33, 399 (1992).
W. C. Willet, M. J. Stampfer, J. E. Manson, G. A. Colditz, F. E. Speizer, B. A. Rosner, L. A. Sampson, C. H. Hennekens, Lancet341, 581 (1993).
A. Pfalzgraf, M. Timm, H. Steinhart, Ernährungswiss.33, 24 (1993).
L. M. Smith, W. L. Dunkley, A. Franke, T. Dairiki, J. Am. Oil Chem. Soc.55, 257 (1978).
L. Deman, J. M. Deman, J. Am. Oil Chem. Soc.60, 1095 (1983).
R. L. Wolff, J. Am. Oil Chem. Soc.71, 277 (1994).
M. Henninger, F. Ulberth, Milchwissenschaft49, 555 (1994).
International IDF Standard 1C:1987, “Milk: Determination of fat content — Röse Gottlieb gravimetric method” (1987).
D. Precht, Fat Sci. Technol.92, 153 (1990).
S. W. Christopherson, R. L. Glass, J. Dairy Sci.52, 1289 (1969).
D. Precht, Kieler Milchwirtsch. Forschungsber.43, 219 (1991).
D. Firestone, P. LaBouliere, J. Assoc. Off. Anal. Chem.48, 437 (1965).
A. C. Lanser, E. A. Emken, J. Am. Oil Chem. Soc.65, 1483 (1988).
F. Ulberth, H.-J. Haider, J. Food Sci.57, 1444 (1992).
E. G. Perkins, T. P. McCarthy, M. A. O’Brien, F. A. Kummerow, J. Am. Oil Chem. Soc.54, 279 (1977).
E. S. van Vleet, J. G. Quinn, J. Chromatogr.151, 396 (1978).
H. Heckers, F. W. Melcher, Am. J. Clin. Nutr.31, 1041 (1978).
Y. Ch. Yoon, E. Renner, Milchwissenschaft37, 197 (1982).
F. Druckrey, C.-E. Høy, G. Holmer, Fat Sci. Technol.87, 350 (1985).
L. Gildenberg, D. Firestone, J. Assoc. Off. Anal. Chem.68, 46 (1985).
A. Strocchi, R. T. Holman, Riv. Ital. Sostanze Grasse48, 617 (1971).
K. Grob Jr.,H. P. Neukom, D. Fröhlich, R. Battaglia, J. High Res. Chromatogr.1, 94 (1978).
K. E. J. Dittmar, H. Heckers, F. W. Melcher, Fat Sci. Technol.80, 297 (1978).
J. Sampugna, L. A. Pallansch, M. G. Enig, M. Keeney, J. Chromatogr.249, 245 (1982).
P. Lund, F. Jensen, Milchwissenschaft38, 193 (1983).
W. M. N. Ratnayake, J. L. Beare-Rogers, J. Chromatogr. Sci.28, 633 (1990).
F. Ulberth, M. Henninger, J. Am. Oil Chem. Soc.69, 829 (1992).
A. Pfalzgraf, M. Timm, H. Steinhart, Z. Ernährungswiss.33, 24 (1993).
T. G. Toschi, P. Capella, C. Holt, W. W. Christie, J. Sci. Food Agric.61, 261 (1993).
W. W. Christie, G. H. McG. Breckenridge, J. Chromatogr.469, 261 (1989).
P. W. Parodi, J. Dairy Sci.59, 1870 (1976).
D. Precht, Z. Ernährungswiss.34, 27 (1995).
D. Precht, J. Molkentin, Int. Dairy J., in press.
J. L. Sebedio, A. Bonpunt, J. Prevost, A. Grandgirard, Fat Sci. Technol.96, 235 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Molkentin, J., Precht, D. Optimized analysis oftrans-octadecenoic acids in edible fats. Chromatographia 41, 267–272 (1995). https://doi.org/10.1007/BF02688039
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02688039