Summary
Human antithrombin III (AT III) is a natural anticoagulant of blood. It is separated from blood preparations by bioselective sorption on sorbents containing heparin as a complementary ligand interacting with AT III. The paper deals with the behaviour of the chromatographic packings obtained by bonding heparin to cross-linked polysaccharide layers deposited on controlled-porosity glass. The properties of the prepared sorbents are discussed with respect to molecular weight of dextran used to form the polysaccharide layers. The results obtained show that when ligand (heparin) and AT III are used the molecular weight of cross-linked dextrans does not significantly influence the chromatographic properties of the affinity packings.
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Ch. Demers, J. S. Ginsberg, P. Henderson, F. A. Ofosu, J. J. Weitz, M. A. Blaichman, Thrombosis and Haemostasis67, 542 (1992).
Ch. Demers, J. S. Ginsberg, M. Johnston, P. Brill-Edwards, A. Panju, Thrombosis and Haemostasis67, 408 (1992).
M. A. Hultin, J. McKay, U. Abildgaard, Thrombosis and Haemostasis59, 468 (1988).
G. E. Vogel, Hämostaseologie3, 54 (1984).
G. E. Vogel, M. v. Clarmann, C. Komm, A. Wirtzfeld, A. Oberdorfer, Intensivmedizin21, 155 (1984).
L. Svendsen, M. Brogli, “Antithrombin III, Biochemistry, function, assay and clinical significance”, E. Wenzel, K. Nienhaus, R. D. Rosenberg (Eds.), Suppl. 3, Ann. Univ. Sarav. Med., 1983, p. 91.
H. E. Karges, N. Heimburger, ibid., p. 105.
M. K. Fagerhol, U. Abildgaard, Scand. J. Haemat.7, 10 (1970).
Hematologia kliniczna (Clinical Haematology), K. Janicki (Ed.), PZWL, Warszawa, 1991, volume 1, p. 199.
F. L. Zhou, D. Muller, X. Santarelli, J. Jozefonvicz, J. Chromatogr.476, 195 (1989).
F. L. Zhou, D. Muller, J. Jozefonvicz, J. Chromatogr.510, 71 (1990).
A. L. Dawidowicz, T. Rauckyte, J. Rogalski, Chromatographia37, 168 (1993).
A. L. Dawidowicz, T. Rauckyte, J. Rogalski, J. Liquid Chromatogr.17, 817 (1994).
K. K. Unger, “Porous Silica — Its Properties and Use as a Support in Column Liquid chromatography”, Elsevier, Amsterdam, 1979.
W. Haller, J. Phys. Chem.42, 686 (1965).
A. L. Dawidowicz, Polish J. Chem66, 313 (1992).
A. L. Dawidowicz, A. Waksmundzki, A. Derylo, Chem. Anal., (Warsaw),24, 965 (1979).
A. L. Dawidowicz, D. Wasilewska, S. Radkiewicz, Adsorp tion Science and Technology. Submitted for publication.
W. H. Scouten, “Affinity Chromatography: Bioselective adsorption on inert matrices”. Volume 59 of Chemical Analysis: A series of monographs on analytical chemistry and its application. P. J. Elving and J. D. Winefordner (Eds.), John Wiley, New York, 1981, p. 48.
Chromatography, 5th edition, fundamentals and application of chromatography and related differential migration methods. Part A: Fundamentals and techniques, edited by E. Heftmann. Journal of Chromatography Library, vol. 51 A. Chapter 7 (T. M. Phillips), p. A 329.
H. Determann, “Gel chromatography, gel filtration, gel permeation, molecular sieves”, 2nd edition, Springer, Berlin, 1969.
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Dawidowicz, A.L. The influence of molecular weight of dextran forming a heparin-bonding polysaccharide layer on the chromatographic properties of sorbents for HPAC analysis of human antithrombin III. Chromatographia 41, 88–93 (1995). https://doi.org/10.1007/BF02688005
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DOI: https://doi.org/10.1007/BF02688005