Summary
A rapid and direct gas chromatographic (GC) method for determining free cholesterol in milk fat using a capillary column and programmed-temperature vaporizer-injector was assayed. It was compared with other procedures involving saponification of fat, solvent extraction of unsaponifiable matter — with and without fractionation by thin-layer chromatography — and transformation of sterols into silyl derivatives prior to GC analysis. This paper proposes an alternative to other published procedures. Repeatability of the method was assessed and the coefficient of variation determined as 2.1 %. The alternative saponification method exhibited comparable accuracy (coefficient of variation=1.8 %). Recovery ranged from 99.1 % to 105.6 %.
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References
H. Glaeser, M Keane, Dairy Industries Int.2, 39 (1992).
R. Sieber, Food Sci. Technol.26, 375 (1993).
M. I. Gurr, “Role of fats in food nutrition”, Elsevier Applied Sci. Publisher, London 1992.
K. Fujimoto, A. Shisikura, N. Kaneda, K. Arai, S. Saitoo, Japanese Patent62, 134042 (1988).
D. G. Oakenfull, R. J. Pearce, G. S. Sidhu, Australian J. Dairy Technol.46, 110 (1991).
G. S. Sidhu, Proceeding Developments in Milk fat Technology Seminar, Dept. Agric. Rural Affairs. Victoria, Australia, Conference Proceeding30, 43 (1989).
S. S. Marschner, J. B. Fine, U.S. Patent4, 505 (1989).
J. Arul, A. Boudreau, J. Makhlouf, R. Tardif, M. R Sahasrabudhe, J. Food Sci.52, 123 (1987).
H. Chen, S. J. Scwartz, G. A. Spanos, J. Dairy Sci.75, 2659 (1992).
A. R. Bhaskar, S. S. H. Rizvi, J. W. Sherbon, J. Food Sci.58, 748 (1993).
W. Xiansheng, T. V. Hung, P. G. Drew, K. Versteeg, Aust. J. Dairy Technol.11, 50 (1990).
International Dairy Federation, Method for the extraction of lipids and liposoluble compounds from milk and dairy products 1E49, Draft FIL-IDF Standard (1990).
L. Alonso, Chromatographia35, 649 (1993).
F. Ulberth, H. Reich, Food Chem.43, 387 (1992).
European Communities, Commission Regulation (EEC) No 3942/92 (1992).
International Dairy Federation, Milk fat and milk fat products. Determination of cholesterol FIL/IDF159, 1992, (1992).
E. Casiraghi, M. Lucisano, C. Pompei, C. Dellea, Milchwissenschaft49, 194 (1994).
R. K. Richardson, J. M. Evers, I. K. Gray, Int. Dairy J.4, 521 (1994).
Ulmann’s Encyclopedia of Industrial Chemistry: Analytical Methods I, vol. 35, Fifth Edition, B. Elvers, S. Hawkins, W. Russey, Eds., VCH, Weinheim, (1994) p. 54.
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Alonso, L., Lozada, L., Fontecha, J. et al. Determination of cholesterol in milk fat by gas chromatography with direct injection and sample saponification. Chromatographia 41, 23–28 (1995). https://doi.org/10.1007/BF02687995
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DOI: https://doi.org/10.1007/BF02687995