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Modified peak compression sampling in fast micro-HPLC

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Summary

The advantages of short microbore columns packed with a sorbent having a particle diameter of 3µm, such as the speed of analysis, the high solute concentration in the effluent and the detectability of small quantities are experimentally verified. The broadening in the valve is eliminated by the peak compression technique reached by adding a ion-pairing agent into the sample. The examples of catecholamines show the dependence of the height equivalent to a theoretical plate on the velocity of the mobile phase. The optimum is h=3 for a column 30mm long, i.d. 0.7mm, particle diameter 3µm. The minimal detectable quantity with a two-electrode amperometric detector with a gold electrode is from 30 to 40fmol for catecholamines.

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, 611 42, Brno, Czechoslovakia

    D. Kouřilová, K. Šlais & M. Krejčí

Authors
  1. D. Kouřilová
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  2. K. Šlais
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  3. M. Krejčí
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Kouřilová, D., Šlais, K. & Krejčí, M. Modified peak compression sampling in fast micro-HPLC. Chromatographia 19, 297–300 (1984). https://doi.org/10.1007/BF02687758

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  • DOI: https://doi.org/10.1007/BF02687758

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