Summary
The resolution of 2-hydroxy acid enantiomers by the principle of ligand exchange chromatography on chemically modified silica gel is described. The phases were synthezised by fixing L-amino acids via 2-glycidoxypropyl-trimethoxysilane to silica gel. The highest enantioselectivity for 2-hydroxy acids was obtained by using L-hydroxyproline as fixed ligand and Cu(II) as complexing agent. A number of aromatic as well as aliphatic 2-hydroxy acids could be resolved on this sorbent.
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Gübitz, G., Mihellyes, S. Direct separation of 2-hydroxy acid enantiomers by high-performance liquid chromatography on chemically bonded chiral phases. Chromatographia 19, 257–259 (1984). https://doi.org/10.1007/BF02687749
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DOI: https://doi.org/10.1007/BF02687749