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Automatic extraction using the mini-column liquid chromatography method for analysis of drugs of abuse in biological fluids

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Summary

The preparation of an automatic extractor using ODS-minicolumn (25mm × 9mm i.d.) for analysis of drugs of abuse in biological fluids and its application are described. The auto-extraction method consists of the sequential operations, adsorption-washing (clean up)-elution(collection of the analyte) and regeneration of column, which continue repeatedly. Recoveries of drugs from spiked plasma and urine samples with the auto-extractor were almost quantitative at the optimum pH and much better than that of the conventional extraction with ether. Reproducibility of the auto-extraction was fairly good and the relative standard deviations of 10 measurements for methamphetamine, THC, morphine, cocaine and methaqualone in urine and plasma were from 0.55% to 4.35%. The simultaneous extractions of methamphetamine, THC, tranquilizers and their metabolites in urine with the autoextractor are presented as applications.

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Authors and Affiliations

  1. National Research Institute of Police Science, 6, Sanban-cho, Chiyoda-ku, P.O. 102, Tokyo, Japan

    Y. Nakahara & H. Sekine

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  1. Y. Nakahara
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  2. H. Sekine
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Nakahara, Y., Sekine, H. Automatic extraction using the mini-column liquid chromatography method for analysis of drugs of abuse in biological fluids. Chromatographia 19, 131–136 (1984). https://doi.org/10.1007/BF02687728

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  • DOI: https://doi.org/10.1007/BF02687728

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