Abstract
Objective and design
In this study, we examined the effect of a single and a repeated hapten-challenge on inflammatory processes in the airways of mice undergoing a hapten-induced non-IgE mediated hypersensitivity reaction.
Methods
BALB/c mice were skin-sensitized with the hapten dinitroflourobenzene (DNFB) and intra-airway challenged with dinitrobenzene sulphonic acid (DNS). Mucosal exudation, tracheal vascular permeability, cellular accumulation, and serum murine mast cell protease (MMCP) were investigated at different time points after the first DNS-challenge and 30 min after a repeated DNS-challenge.
Results
MMCP levels in serum were increased at all time points after single challenge and repeated challenge. Increased vascular permeability as determined by Monastral blue staining, was found in the trachea of DNFB-sensitized mice after single DNS-challenge. A second exposure to DNS profoundly enhanced the Monastral blue labeling of the tracheal blood vessels of DNFB-sensitized mice. Furthermore, increased mucosal exudation and polymorphonuclear cell (PMN) accumulation were present in DNFB-sensitized mice compared to vehicle-sensitized animals after the first DNS challenge.
Conclusions
Increased mucosal exudation, vascular permeability, and PMN accumulation are prominent inflammatory features of the DNFB-induced hypersensitivity reaction in the airways. Furthermore, mast cell activation is associated with this hapten-induced hypersensitivity reaction.
Similar content being viewed by others
References
Dearman RJ, Kimber I. Differential stimulation of immune function by respiratory and contact chemical allergens. Immunology 1991; 72: 563–70.
Scheerens H, Buckley TL, Davidse EM, Garssen J, Nijkamp FP, Van Loveren H. Toluene diisocyanate-induced in vitro tracheal hyperreactivity in the mouse. Am J Respir Crit Care Med 1996; 154: 858–65.
Buckley TL, Nijkamp FP. Airways hyperreactivity and cellular accumulation in an delayed-type hypersensitivity reaction in the mouse: Modulation by capsaicin-sensitive nerves. Am J Respir Crit Care Med 1994; 149: 400–7.
Buckley TL, Nijkamp FP. Mucosal exudation associated with a pulmonary delay-type hypersensitivity reaction in the mouse. J Immunol 1994; 153: 4169–78.
Garssen J, Nijkamp FP, Wagenaar SS, Zwart A, Askenase PW, Van Loveren H. Regulation of delayed-type hypersensitivity-like responses in the mouse lung, determined with histological procedures: serotonin, T-cell suppressor-inducer factor and high antigen dose tolerance regulate the magnitude of T-cell dependent inflammatory reactions. Immunology 1989; 68: 51–8.
Askenase PW, Van Loveren H, Kraeuter-Kops S, Ron Y, Meade R, Theoharides TC et al. Defective elicitation of delayed-type hypersensitivity in W/Wv and SI/SId mast cell-deficient mice. J Immunol 1983; 131: 2687–94.
Kraneveld AD, Muis T, Koster AS, Nijkamp FP. Role of mucosal mast cells in early vascular permeability changes of intestinal DTH reaction in the rat. Am J Physiol 1998; 274: G832–9.
Garssen J, Nijkamp FP, Van Vugt E, Van der Vliet H, Van Loveren H. T cell-derived antigen binding molecules play a role in the induction of airway hyperresponsiveness. Am J Respir Crit Care Med; 150: 1528-38.
van Loveren H, Meade R, Askenase PW. An early component of delayed-type hypersensitivity mediated by T cells and mast cells. J Exp Med 1983; 157: 1604–17.
Baluk P, Thurston G, Murphy TJ, Bunnett NW, McDonald DM. Neurogenic plasma leakage in mouse airways. Br J Pharmacol 1999; 126: 522–8.
Rogers DF, Boschetto P, Barnes PJ. Plasma exudation: correlation between Evans Blue dye and radiolabeled albumin in guinea pig airways in vivo. J Pharmacol Methods 1989; 21: 309–15.
Redegeld FA, Garssen J, Van Loveren H, Koster AS, Nijkamp FP. Chararization of the binding of a DTH initiating T-cell derived factor to mast cells. FASEB J 1994; 8: A981.
Redegeld FAM, Kraneveld AD, Nijkamp FP. The role of mast cells in non-IgE mediated asthma. Eur Respir Rev 2000; 10: 307–8.
Kraneveld AD, Buckley TL, van Heuven-Nolsen D, van Schaik Y, Koster AS, Nijkamp FP. Delayed-type hypersensitivity-induced increase in vascular permeability in the mouse small intestine: inhibition by depletion of sensory neuropeptides and NK1 receptor blockade. Br J Pharmacol 1995; 114: 1483–9.
Kudo C, Yamashita T, Araki A, Terashita M, Watanabe T, Atsumi M et al. Modulation of in vivo immune response by selective depletion of neutrophils using a monoclonal antibody, RP-3. I. Inhibition by RP-3 treatment of the priming and effector phases of delayed type hypersensitivity to sheep red blood cells in rats. J Immunol 1993; 150: 3728–38.
Gao JX, Issekutz AC, Issekutz TB. Neutrophils migrate to delayed-type hypersensitivity reactions in joints, but not in skin. Mechanism is leukocyte function-associated antigen-1-/Mac-1-independent. J Immunol 1994; 153: 5689–97.
Buchanan KL, Murphy JW. Kinetics of cellular infiltration and cytokine production during the efferent phase of a delayed-type hypersensitivity reaction. Immunology 1997; 90: 189–97.
van Houwelingen A, van der Avoort LA, Heuven-Nolsen D, Kraneveld AD, Nijkamp FP. Repeated challenge with dinitrobenzene sulphonic acid in dinitrofluorobenzene-sensitized mice results in vascular hyperpermeability in the trachea: a role for tachykinins. BrJ Pharmacol 1999; 127: 1583–8.
Baluk P, Bowden JJ, Lefevre PM, McDonald DM. Upregulation of substance P receptors in angiogenesis associated with chronic airway inflammation. Am J Physiol 1997; 273: L565–71.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
van Houwelingen, A.H., de Jager, S.C.A., Kool, M. et al. Hypersensitivity reactions in mouse airways after a single and a repeated hapten challenge. Inflamm Res 51, 63–68 (2002). https://doi.org/10.1007/BF02684001
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02684001