Abstract
A method for the isolation of milligram quantities of 3 minorAlternaria mycotoxins, altenuisol (AS), altertoxin I (AT-I) and altertoxin II (AT-II) was developed. Crude toxin preparation was first subjected to a preparative high pressure liquid Chromatograph step using PrepLC/System 500 and a silica gel column. The 3 minor toxins were eluted from the column with 25% ethyl acetate. Further purification of these toxins was achieved by passing the partially purified toxins through a Sephadex LH-20 column (2.5 X 25 cm) twice. In a solvent system of hexane/methylehe chloride/methanol (1:1:1, v/v/v), AT-I was eluted from the Sephadex column first, followed by AT-II and AS. The distribution of variousAlternaria mycotoxins in different fractions obtained from the Sephadex step and some of the physicochemical properties of AT-I and AT-II are described.
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Chu, F.S. Isolation of altenuisol and altertoxins I and II, minor mycotoxins elaborated byalternaria . J Am Oil Chem Soc 58, A1006–A1008 (1981). https://doi.org/10.1007/BF02679313
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DOI: https://doi.org/10.1007/BF02679313