Abstract
A proposed rapid and versatile method of analysis for aflatoxins in cottonseed products using Waters Associates’ Sep- Pak silica cartridge clean- up is presented. Samples are extracted with acetonitrile/ water azeotrope and a filtered aliquot is evaporated to dryness. A toluene/acetonitrile (96:4) solution of the dry extract is applied to a Sep- Pak column. Gossypol derivatives and other artifacts are eluted with chloroform/ethyl ether (1:4). The Sep- Pak is then mated with either a Romer minicolumn for rapid minicolumn application or a small column of acid alumina deactivated with 4% water to produce a clean extract for quantitation by thin layer chromatography or high pressure liquid chromatography.
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References
Pons, W.A., Jr., J. Assoc. Off. Anal. Chem. 58:746 (1975).
Velasco, J., Ibid. 53:611 (1970).
Romer, T.R., Ibid. 58:500 (1975).
“Official Methods of Analysis of the Assoc. Off. Anal. Chem.,” 13th Edn., AOAC, Washington, DC, 1980, Chapt, 26, Sec. 26.019.
Pons, W.A., Jr., A.F. Cucullu, A.O. Franz, Jr., and L.A. Gold- blatt, JAOCS 45:694 (1968).
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Mckinney, J.D. Rapid analysis for aflatoxins in cottonseed products with silica gel cartridge clean- up. J Am Oil Chem Soc 58, A935–A937 (1981). https://doi.org/10.1007/BF02679293
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DOI: https://doi.org/10.1007/BF02679293