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In vitro studies of Anti-Inflammatory agents and prostaglandin e2 effects on stimulated normal human fibroblast cultures

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Lanas A, Haggerty P, Hirschowitz BI. In vitro studies of anti-inflammatory agents and prostaglandin E effects on stimulated normal human fibroblast cultures. Inflammopharmacology. 1994;2:377-387. To study the mechanisms whereby anti-inflammatory agents and prostaglandins may affect wound healing (fibroblast proliferation), we studied normal human fibroblasts growth in short-term, 24 h, culture by13H-thymidine incorporation and longer term 6–14 day growth in cell numbers by the crystal violet method. Therapeutic doses of 5-aminosalicylic acid (5-ASA) but not ibuprofen increase fibroblast proliferation stimulated for 24 h by platelet-derived growth factor (PDGF-AB). Both dexamethasone and prostaglandin-E2 strongly suppressed fibroblast growth but the effects were not additive. PDGF stimulated PGK2 production from fibroblasts and our data suggest that prostaglandins may act as negative Feedback on growth factor mitogenesis. Long-term fibroblast culture was inhibited by ibuprofen but not 5-ASA. We conclude that these effects may explain, at least in part, the clinical actions of these drugs on some pathological conditions and wound healing.

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  1. Division of Gastroenterology, University of Alabama at Birmingham, 35294, AL, USA

    A. Lanas, P. Haggerty & B. I. Hirschowitz

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  1. A. Lanas
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  2. P. Haggerty
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  3. B. I. Hirschowitz
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Lanas, A., Haggerty, P. & Hirschowitz, B.I. In vitro studies of Anti-Inflammatory agents and prostaglandin e2 effects on stimulated normal human fibroblast cultures. Inflammopharmacology 2, 377–387 (1994). https://doi.org/10.1007/BF02678604

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