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The spleen ofmustelus schmitti (chondrichthyes, triakidae): A light and electron microscopic study

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Abstract

The spleen ofMustelus schmitti is described, being an elongated organ running dorsally along the stomach and surrounded by a thin capsule without muscular tissue. The classical division between red and white pulps was evident, with a marginal sinus surrounding the latter. Numerous ellipsoids were located in the red pulp, inside nodular-like structures, or in the marginal sinus. Two types of reticular cells were apparent as well as macrophages and melanomacrophages. Hemopoiesis was present through immature and mature cells of the erythroid and thrombocytoid lineages, but no evidence of granulopoiesis was found. Comparison amongMustelus species and between chondrichthyan and mammalian spleens are made.

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Literature Cited

  • Agius, C. and S. S. Agbede. 1984. An electron microscopic study on the genesis of lipofuchsin, melanin and haemosiderin in the haemopoietic tissue of fish. J. Fish Biol., 24: 471–488.

    Article  CAS  Google Scholar 

  • Awaya, K.. 1986. Phylogeny of lymphoreticular system in lower vertebrates. Recent Adv. Reticuloendothel. System. Res. 21: 1–29.

    Google Scholar 

  • Blue, J. and L. Weiss. 1981. Species variation in the structure and function of the marginal zone—an electron microscope study of cat spleen. Am. J. Anat., 161: 169–187.

    Article  PubMed  CAS  Google Scholar 

  • Daimon, T. and K. Uchida. 1985. Ultrastructural evidence of the existence of the surface connected canalicular system in the thrombocyte of the sharkTriakis scyllia. J. Anat., 141: 193–200.

    PubMed  CAS  Google Scholar 

  • Emery, S.. 1986. Hematological comparisons of endothermicvs ectothermic elasmobranch fishes. Copeia, 1986: 700–705.

    Article  Google Scholar 

  • Espenes, A., C. M. Press, B. H. Dannevig and T. Landsverk. 1995. Investigation of the structural and functional features of splenic ellipsoids in rainbow trout (Oncorhynchus mykiss). Cell Tissue Res., 279: 469–474.

    Google Scholar 

  • Fänge, R.. 1977. Size relation on lymphomyeloid tissue in the elasmobranchs. Acta Zool. (Stockh.), 58: 125–128.

    Google Scholar 

  • Fänge, R.. 1987. Lymphomyeloid tissues and blood cell morphology in elasmobranchs. Arch. Biol. (Bruxelles), 98: 187–208.

    Google Scholar 

  • Fänge, R. and S. Nilsson. 1985. The fish spleen: structure and function. Experientia 41: 152–158.

    Article  PubMed  Google Scholar 

  • Galíndez, E. J. and M. C. Aggio. 1995. Hematological parameters and blood cell morphology ofMustelus schmitti (Chondrichthyes, Triakidae). An. Mus. Hist. Nat. Valparaiso, 23: 1–9.

    Google Scholar 

  • Galíndez, E. J. and M. C. Aggio. 1996. Morformetria del complejo linformieloide deMustelus schmitti (Chondrichthyes, Triakidae). Iheringia, sér. Zool., 80: 135–142.

    Google Scholar 

  • Galíndez, E. J. and M. C. Aggio. 1997. The hemopoietic system: a phylogenetic approach. Histol. Histopathol., 12: 823–826.

    PubMed  Google Scholar 

  • Gordon, M. Y.. 1991. Hemopoietic growth factors and receptors: bound and free. Cancer Res. 3: 127–133.

    CAS  Google Scholar 

  • Hartwig, H. and H. G. Hartwig. 1985. Structural characteristics of the mammalian spleen indicating storage and release of red blood cells. Aspects of evolutionary and environmental demands. Experientia 41: 159–163.

    Article  PubMed  CAS  Google Scholar 

  • Herraez, M. P. and A. Zapata. 1991. Structural characterization of the melano-macrophages centres (MMC) of goldfishCarassius auratus. Eur. J. Morphol., 29: 89–102.

    PubMed  CAS  Google Scholar 

  • Hine, P. M. and J. M. Wain. 1987. Composition and ultrastructure of elasmobranch granulocytes. III. Sharks (Lamniformes). J. Fish Biol. 30: 567–576.

    Article  Google Scholar 

  • Honma, Y., K. Okabe and A. Chiba. 1984. Comparative histology of the Leydig and epigonal organs in some elasmobranchs. Japan. J. Ichthyol., 31: 47–54.

    Google Scholar 

  • Hyder, S. L., L. M. Cayer and C. L. Pettey. 1983. Cell types in peripheral blood of the nurse shark: an approach to structure and function. Tissue & Cell, 15: 437–455.

    Article  CAS  Google Scholar 

  • Jordan, H. E. and C. C. Speidel. 1924. Studies on lymphocytes. II. The origin, function and fate of the lymphocytes in fishes. J. Morphol., 38: 529–549.

    Article  Google Scholar 

  • Kanesada, A.. 1956. A phylogenetical survey of hemocytopoietic tissues in submammalian vertebrates. Bull. Yamaguchi Med. Sch., 4: 1–35.

    Google Scholar 

  • Kobayashi, K., S. Tomonaga, K. Teshima and T. Kajii, 1985. Ontogenic studies on the appearance of two classes of immunoglobulin-forming cells in the spleen of the Aleutian skate,Bathyraja aleutica, a cartilaginous fish. Eur. J. Immunol., 15: 952–956.

    Article  PubMed  CAS  Google Scholar 

  • Morrow, W. J. W. and A. Pulsford. 1980. Identification of the peripheral blood leukocytes of the dogfish (Scyliorhinus canicula L.) by electron microscopy. J. Fish Biol., 17: 461–475.

    Article  Google Scholar 

  • Murata, H.. 1959. Comparative studies of the spleen in submammalian vertebrates. II. Minute structure of the spleen, with special reference to the periarterial lymphoid sheat. Bull. Yamaguchi Med. Sch., 6: 83–105.

    Google Scholar 

  • Oguri, M.. 1983. On the Leydig organ in the oesophagus of some elasmobranchs. Bull. Jpn. Soc. Sci. Fisheries, 50: 1381–1384.

    Google Scholar 

  • Pulsford, A., R. Fänge and W. J. W. Morrow. 1982. Cell types and interactions in the spleen of the dogfishScyliorhinus canicula L.: an electron microscopic study. J. Fish Biol., 21: 649–662.

    Article  Google Scholar 

  • Pulsford, A., R. Fänge and A. Zapata. 1991. The thymic microenvironment of the common sole,Solea solea. Acta Zool. (Stockh.), 70: 209–216.

    Article  Google Scholar 

  • Pulsford, A. and A. Zapata. 1989. Macrophages and reticulum cells in the spleen of the dogfishScyliorhinus canicula L Acta Zool. (Stockh.), 70: 221–227.

    Google Scholar 

  • Quesenberry, P., R. B. Crittenden, P. Lowry, E. W. Kittler, S. Rao, S. Peters, H. Ramshaw and F. Steward. 1994.In vitro andin vivo studies of stromal niches. Blood Cells, 20: 97–106.

    PubMed  CAS  Google Scholar 

  • Shepro, D., F. A. Belamarich and R. Branson. 1966. The fine structure of the thrombocyte in the dogfish (Mustelus canis) with special reference to microtubule orientation. Anat. Rec., 156: 203–214.

    Article  PubMed  CAS  Google Scholar 

  • Stokes, E. E. and B. G. Firkin. 1971. Studies on the peripheral blood of the Port Jackson shark (Heterodontus portusjacksoni) with particular reference to the thrombocyte. Br. J. Haematol., 20: 427–435.

    PubMed  CAS  Google Scholar 

  • Tablin, F. and L. Weiss. 1985. Equine bone marrow: a quantitative analysis of erythroid maturation. Anat. Rec., 213: 202–206.

    Article  PubMed  CAS  Google Scholar 

  • Tavassoli, M.. 1993. Hematopoietic survival factors. Exp. Hematol., 21: 1511–1513.

    PubMed  CAS  Google Scholar 

  • Weiss, L.. 1991. Barrier cells in the spleen. Immunology Today, 12: 24–29.

    Article  PubMed  CAS  Google Scholar 

  • Weiss, L. and U. Geduldig. 1991. Barrier cells: stromal regulation of hematopoiesis and blood cell release in normal and stressed murine bone marrow. Blood, 78: 975–990.

    PubMed  CAS  Google Scholar 

  • Yoffey, J. M.. 1929. A contribution to the study of the comparative histology and phylogeny of the spleen, with reference chieffy to its cellular constituents. 1. In fishes. J. Anat., 63: 314–344.

    PubMed  CAS  Google Scholar 

  • Zapata, A.. 1980a. Ultrastructure of elasmobranch lymphoid tissue. I. Thymus and spleen. Dev. Comp. Immunol., 4: 459–473.

    Article  PubMed  CAS  Google Scholar 

  • Zapata, A.. 1980b. Splenic erythropoiesis and thrombopoiesis in Elasmobranchs: an ultrastructural study. Acta Zool. (Stockh.), 61: 59–64.

    Google Scholar 

  • Zapata, A. and E. Cooper. 1990. The spleen. Pages 150–218in The immune system: comparative histophysiology. John Wiley, Chichester.

    Google Scholar 

  • Zapata, A., M. Torroba, A. Vicente, A. Varas, R. Sacedón and E. Jiménez. 1995. The relevance of cell microenvironments for the appearance of lymphoid-haemopoietic tissues in primitive vertebrates. Histol. Histopathol., 10: 761–778.

    PubMed  CAS  Google Scholar 

  • Zipori, D.. 1988. Hemopoietic microenvironments. Pages 27–62in N. G. Testa and N. P. Gale, eds. Hematopoiesis, 1st ed. Marcel Dekker, New York.

    Google Scholar 

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Galíndez, E.J., Aggio, M.C. The spleen ofmustelus schmitti (chondrichthyes, triakidae): A light and electron microscopic study. Ichthyological Research 45, 179–186 (1998). https://doi.org/10.1007/BF02678560

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