Abstract
Dipole moments of the fluorescent probes 1-phenylnaphthylamine (1-AN) and 1-anilinonaphthalene-8-sulfonate (1,8-ANS) are measured using electro-optical absorption and emission methods. Dipole moments in the ground and excited states were measured in cyclohexane and dioxane. It is shown that the charge distributions in the 1-AN and 1,8-ANS molecules differ substantially. The spectral dependence of the electro-optical coefficients suggests that the absorption spectrum of 1,8-ANS is due to a superposition of (at least two) electronic transitions. It is found that spectra of 1-AN in erythocyte ghosts are inhomogeneously broadened. The above effect makes it possible to selectively excite probe molecules localized at different sites of a membrane. Dielectric interactions (described by the local dielectric constant) are investigated in human erythrocyte membranes. It is found that the dielectric constant of erythrocyte membranes varies from 6.79 ±0.8 to 17.6±3.5 depending on the excitation frequency and, therefore, on the localization of the probe.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 66, No. 3, pp. 387–393, May–June, 1999.
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Nemkovich, N.A., Baumann, W., Kruchenok, Y.V. et al. Use of the red-edge excitation effect for investigation of dielectric interactions in biomembranes. J Appl Spectrosc 66, 415–422 (1999). https://doi.org/10.1007/BF02676774
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DOI: https://doi.org/10.1007/BF02676774