Abstract
A murine monoclonal antibody HAb27 specific for human hepatocarcinoma has been developed for radio-immunolocalization in animal models. The isotype of this antibody was IgG1, κ. In the present study, we used a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable region genes by the polymerase chain reaction. Sequence analysis of the heavy variable region indicated that the VH region was highly homologous to the plasmacytoma cell line MOPC21 gene, and closely related to germline genes of the VHIII family. The JH region was encoded by the JH3 gene. For the light chain, the Vκ segment of the antibody showed the highest homology to the germline VκOx1 gene, and the Jκ region was Jκ5.
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References
Reynolds, JC, Del Vecchio S, Sakahara H, et al. Antimurine response to mouse monoclonal antibodies: Clinical findings and implications. Nucleic Med Biol 1989; 16:121.
Morrison SL, Johnson MJ, Herzenberg LA, et al. Chimeric human antibody molecules: Mouse antigen-binding domains with human constant region domains. Proc Natl Acad Sci USA 1984; 81:6851.
Batra SK, Niswonger ML, Wikstrand CJ, et al. Mouse/human chimeric Mel-14 antibody: Genomic cloning of the variable region genes, linkage to human constant region genes, expression and characterization. Hybridoma 1994; 13:87.
Riechmann L, Clark M, Waldman H, et al. Reshaping human antibodies for therapy. Nature 1988; 332:323.
Lewis AP, Growe JS. Immunoglobulin complementary-determining region grafting by recombinant polymerase chain reaction to generate humanised monoclonal antibodies. Gene 1991; 101:297.
Colcher D, Bird R, Roselli M, et al.In vivo tumor targeting. of a recombinant single-chain antihgen-binding protein. J Natl Cancer Inst 1990; 82:1191.
Milenic DE, Yokota T, Filpula DR, et al. Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res 1991; 51:6363.
Hu CM, Liu YF, Sui YF, et al. Preparation and characterization of two clones of monoclonal antibodies against hepatocellular carcinoma. J Monoclonal Antibody 1994; 10:16.
Sambrook J, Fritsch EF, Maniatis T. In molecular cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 1990.
Lawler AM, Lin PS, Gearhart PJ. Adult B-cell repertoire is biased toward two heavy-chain variable-region genes that rearrange frequently in fetal pre-B cells. Proc Natl Acad Sci USA. 1987; 84:2454.
Kaartinen M, Makela O. Functional analogues of the VκOx1 gene in different strains of mice: Evolutionary conservation but diversity based V-J joining. J Immunol 1987; 138:1613.
French DL, Laskov R, Scharff MD. The role of somatic hypermutation in the generation of antibody diversity. Science 1989; 244:1152.
Thammara P, Gaito AL, Largen MT. Sequences of variable regions of a monoclonal antiboey specific to the thyroid hormone, triiodo-L-thyronine. Molecular Immunol 1992; 29:1027.
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Ping, Y., Lei, G., Chuanmin, H. et al. Cloning and sequencing of immunoglobulin variable-region gene of a monoclonal antibody specific for human hepatocarcinoma. Chinese Journal of Cancer Research 8, 1–4 (1996). https://doi.org/10.1007/BF02674956
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DOI: https://doi.org/10.1007/BF02674956