Chinese Journal of Cancer Research

, Volume 7, Issue 4, pp 275–279 | Cite as

Hepatitis B and C viruses and their interaction in the pthogenesis of hepatocellular carcinoma

  • Wang Shulin
  • Zhang Yaozheng
Clinical Observations


The prevelence of hepatitis B and C virus (HBV and HCV) infections was studied in 46 patients with hepatocellular carcinoma (HCC) and 30 controls without liver disease. In addition to viral serological markers, Serum hepatitis B virus DNA and hepatitis C virus RNA were determined with a nested polymerase chain reaction assay. The positive rates of HBV-DNA and HBV serological markers were 69.5% and 76.1% respectively, significantly higher than that in controls (16.7% and 16.7%,P<0.01); 14 patients (30.4%) were HCV-RNA positive, and 12 patients were Anti-HCV positive, remarkablely higher than that in controls, too. (6.7% and 6.7% respectively,P<0.05). Serum HBV-DNA were all positive in HCC patients with serum HBeAg and/or HBcAb positive. Among HCC, 20% of HBV seronegative cases had HBV-DNA detectable in their serum. Coinfection by the two viruses was detected in seven subjects (15.2%), but no clinical differences were found between these and the rest of patients. We concluded that nearly 95.6% (44 of 46 patients studied) of cases of HCC in our geographical area are related to hepatitis infections. Hepatitis B virus is more prevelent than hepatitis C virus in patients with HCC, and the infection is still active when the tumor is diagnosed. This fact is probably important in the contribution of hepatitis B and C virus infections to the development of hepatocellular carcinoma.

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  1. 1.
    Tswana SA and Moyo SR. The Interrelationship between HBV-markers and HIV Antibodies in Patients with Hepatocellular Carcinoma. J Med Virol 1992; 37: 161.PubMedCrossRefGoogle Scholar
  2. 2.
    Maureen C Hatch, Chen Chienjen, Bruce Levin, et al. Urinary Aflatoxin Levels, Hepatitis B Virus Infection and Hepatocellular Carcinoma in Taiwan. Intl J Cancer 1993; 54: 931.CrossRefGoogle Scholar
  3. 3.
    Liang TJ, Isselbacher KJ, Wands JR. Rapid Identification of Low Level Hepatitis B Related Viral Genome in Serum. J Clin Invest 1989; 84: 1367.PubMedCrossRefGoogle Scholar
  4. 4.
    Kaneko S, Miller RH, Feinstone SW, et al. Detection of Serum Hepatitis B Virus DNA using The Polymerase Chain Reaction Assay. Proc Natl Acad Sci USA 1989; 86: 312.PubMedCrossRefGoogle Scholar
  5. 5.
    Choo QL, Kuo G, Weiner AJ, et al. Isolation of cDNA Clone Derived From A Blood-Borne Non-A, Non-B Viral he Hepatitis Genome. Science 1989; 244: 359.PubMedCrossRefGoogle Scholar
  6. 6.
    Colombo M, Kuo G, choo QL, et al. Prevelence of Antibodies To Hepatitis C Virus In Italian Patients With Hepatocellular Carcinoma. Lancet 19891 2: 1006.Google Scholar
  7. 7.
    Ruiz J, Sangro B, Caende JI, et al. Hepatitis B and C Viral Infactions In Patients With Hepatocellular Carcinoma. Hepatology 1992; 16: 637.PubMedCrossRefGoogle Scholar
  8. 8.
    Chomczynski P, et al. Single-step Method of RNA Isolation By Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction. Annal Biochem 1987; 162: 156.CrossRefGoogle Scholar
  9. 9.
    Ayoola EA, Odelola HA, Johnson AOK. Primary Liver Cancer (PLC) after Non-A, Non-B Hepatitis (NANBBH)(Abstract) Hepatology 1982; 2: 154.Google Scholar
  10. 10.
    Resnick RH, Stone K, Antorioli D. Primary Hepatocellular Carcinoma Following Non-A, Non-B Posttransfusion Hepatitis. Dig Dis Sci 1983; 28: 908.PubMedCrossRefGoogle Scholar
  11. 11.
    Sakamoto M, Hirohashi S, Tsuda H, et al. Increasing Incidence of Hepatocellular Carcinoma Possibly Associated with Non-A, Non-B Hepatitis in Japan Disclosed by Hepatitis B Virus DNA Analysis of Surgically Resected Cases. Cancer Res 1988; 48: 7294.PubMedGoogle Scholar
  12. 12.
    Kuo G, Choo QL, Alter HJ, et al. An Assay For Circulating Antibodies to A Major Etiologic Virus of Non-A, Non-B Hepatitis. Science 1989; 244: 362.PubMedCrossRefGoogle Scholar
  13. 13.
    Farci P, alter HJ, Wong D, et al. A Long Term Study of Hepatitis C Virus Infection In Non-A, Non-B Hepatitis. N Engl J Med 1991; 25: 98.CrossRefGoogle Scholar
  14. 14.
    Thiers V, Nakajima E, Krensdorf D, et al. Detection and Cloning By The Polymerase Chain Reaction of Hepatitis B Virus DNA Sequences In Blood Samples Negative For HBV Serological Markers. Lancet 1988; 2: 1273.PubMedCrossRefGoogle Scholar
  15. 15.
    Kaneko S, Miller RH, Feinstone SM, et al. detection of Hepatitis B Virus DNA Using The Polymerase Chain reaction assay. Proc Natl Acad USA 1989; 86: 312.CrossRefGoogle Scholar
  16. 16.
    Lai MY, Chen PJ, Yang DM, et al. Identification And Characterization Of Intrahepatic Hepatitis B Virus DNA In HBsAg-sreonegative patients with Chronic Liver Disease And Hepatocellular Carcinoma In Tyaiwan. Hepatology 1990; 12: 575.PubMedCrossRefGoogle Scholar
  17. 17.
    Peterlini P, Gerken G, Nakajima E, et al. Polymerase Chain Reaction To Detect Hepatitis B Virus DNA And RNA Sequences In Primary Liver Cancers Form Patients Negative For Hepatitis B Surface Antigen. N Engl J Med 1990; 323: 80.CrossRefGoogle Scholar
  18. 18.
    Jonathan L Brawn, William F Carman, Howard C Thomas. The Clinical Significance of Molecular Variation Within The Hepatitis B Virus Genome. Hepatology 1992; 15: 144.CrossRefGoogle Scholar
  19. 19.
    J Michnel Bioshop. The Molecular genetics Of Cancer. science 1987; 235: 305.Google Scholar
  20. 20.
    Tokino t, Fukushige S, Nakamura T, et al. Chromosomal Translocation And Inverted Duplication Associated with Integrated Hepatitis B Virus In Hepatocellular Carcinoma. J Virol 1987; 61: 3848.PubMedGoogle Scholar
  21. 21.
    Takada S, Koide K. Trans-Activation Function of a 3' Truncated X Gene-C Fusion Product From In Tegrated Hepatitis B Virus DNA In Chromic Hepatitis Tissues. Proc Natl Acad Sci USA 1990; 87: 5628.PubMedCrossRefGoogle Scholar
  22. 22.
    Liang TJ, Baruch Y, ben-porath E, et al. Hepatitis B Virus Infection In Patients With Idiopathic Liver disease. Hepatology 1991; 13: 1044.PubMedGoogle Scholar
  23. 23.
    Chwenming Shih, Szecheng J LO, et al. Suppression of Hepatitis B Virus Expression and Replication By Hepatitis C Virus core Protein In HuH-7 Cells. J Virol 1993; 67(10): 5823.PubMedGoogle Scholar

Copyright information

© Chinese Journal of Cancer Research 1995

Authors and Affiliations

  • Wang Shulin
    • 1
  • Zhang Yaozheng
    • 1
  1. 1.Department of Clinical BiochemistryDalian Medical UniversityDalian

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