Abstract
Vimentin was isolated and purified from the pig eye lens by homogenization, ultracentrifugation, extraction in urea buffer and preparative eletrophoresis. It was identified with SDS-PAGE and rabbit anti-vimentin was raised against the purified vimentin. The specificity of anti-vimentin was examined with immunohistochemical technique and double immune diffusion. Results showed that the vimentin antibody possessed good specificity for mesenchyme-derived cells. Tumor tissue sections from 151 cases were stained with anti-vimentin, anti-keratin, anti-desmin, anti-S-100 protein, anti-Factor-FV III related anti-gen, and anti-lysozyme. Positive staining was obtained in mesenchyme-derived cells, while the epithelial tumor cells did not react with anti-vimentin. It indicated that vimentin anti-body is effective for tumor differential diagnosis in surgical pathology.
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Nong, Z., Yuee, Z., Zhongnian, C. et al. Vimentin and tumor diagnosis. Chinese Journal of Cancer Research 3, 42–45 (1991). https://doi.org/10.1007/BF02672089
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DOI: https://doi.org/10.1007/BF02672089