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The preliminary application of in situ hybridization in detecting proto-oncogenes expression in human leukemic cells

  • Basic Investigations
  • Published:
Chinese Journal of Cancer Research

Abstract

An in situ hybridization technique with35S labelled proto-oncogene probes (c-myc & c-fes) was used to detect their expression in bone marrow cells of 22 cases of leukemia of various types and immature granulocytes and erythroblasts of 16 nomal myelograms as controls. Both c-myc and c-fes were detectable in leukemic cells as well as in immature granulocytes and erythroblasts of normal bone marrow, but the expression extent varied in different cases. The levels of c-myc expression in leukemic cells were higher than those in controls (P<0.001). There was no difference of c-fes expression in two groups of bone marrow cells (P>0.05). This technique provides us a new method in studying variations of proto-oncogene expression in leukemic cells.

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Authors and Affiliations

  1. Institute of Haematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai, China

    Zhao Lian, Peng Miao, Du Xinxu, Chen Shurong, Cai Jingren, Li Xiusong, Zhang Fenqin & Wang Zhenyi

  2. Department of Biochemistry, Shanghai Second Medical University, Shanghai, China

    Wang Dunrui, Wang Xiaogang & Chen Shishu

Authors
  1. Zhao Lian
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  2. Peng Miao
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  3. Du Xinxu
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  4. Chen Shurong
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  5. Cai Jingren
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  6. Li Xiusong
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  7. Zhang Fenqin
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  8. Wang Zhenyi
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  9. Wang Dunrui
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  10. Wang Xiaogang
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  11. Chen Shishu
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Lian, Z., Miao, P., Xinxu, D. et al. The preliminary application of in situ hybridization in detecting proto-oncogenes expression in human leukemic cells. Chinese Journal of Cancer Research 3, 15–17 (1991). https://doi.org/10.1007/BF02672083

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  • DOI: https://doi.org/10.1007/BF02672083

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