Nuclear DNA contents of more than 100 important plant species were measured by flow cytometry of isolated nuclei stained with propidium iodide.Arabidopsis exhibits developmentally regulated multiploidy and has a 2C nuclear DNA content of 0.30 pg (145 Mbp/1C), twice the value usually cited. The 2C value for rice is only about three times that ofArabidopsis. Tomato has a 2C value of about 2.0 pg, larger than commonly cited. This survey identified several horticultural crops in a variety of families with genomes only two or three times as large asArabidopsis; these include several fruit trees (a pricot, cherry, mango, orange, papaya, and peach). The small genome sizes of rice and the horticultural plants should facilitate molecular studies of these crops.
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DNA content of the unreplicated haploid chromosome complement
chicken red blood cells
Arumuganathan, K. and E.D. Earle 1991. Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Reporter 9:221–231.
Bennett, M.D. 1985. Intraspecific variation in DNA amount and the nucleotypic dimension in plant genetics. In:Plant Genetics (ed. M. Freeling), pp. 283–302. Alan R. Liss, New York.
Bennett, M.D. and J.B. Smith. 1976. Nuclear DNA amounts in angiosperms. Phil. Trans. R. Soc. Lond. B 274:227–274.
Bennett, M.D., J.B. Smith and J.S. Heslop-Harrison. 1982. Nuclear DNA amounts in angiosperms. Proc. R. Soc. Lond. B 216:179–199.
Cameron, M.J. 1990. Macintosh graphics for the EPICS flow cytometer user. Cytometry 11:916–918.
De Rocher, E.J., K.R. Harkins, D.W. Galbraith and H.J. Bohnert. 1990. Developmentally regulated systemic endopolyploidy in succulents with small genomes. Science 250:99–101.
Galbraith, D.W., K.R. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma and E. Firoozabady. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1051.
Galbraith, D.W. 1990. Flow cytometric analysis of plant genomes. In:Methods in Cell Biology (eds. Z. Datzynkiewicz and H. Crissman), pp. 549–563. Academic Press, New York.
Iyengar, G.A.S. and S.K. Sen. 1978. Nuclear DNA content of several wild and cultivatedOryza species. Environ. Exp. Bot. 18:219–224.
Leutwiler, L.S., B.R. Hough-Evans and E.M. Meyerowitz. 1984. The DNA ofArabidopsis thaliana. Mol. Gen. Genet. 194:15–23.
Laurie, D.A. and M.D. Bennett. 1985. Nuclear DNA content in the generaZea andSorghum. Intergeneric, interspecific and intraspecific variation. Heredity 55:307–313.
Messeguer, R., M.W. Ganal, J.C. Steffens and S.D. Tanksley. 1991. Characterization of the level, target sites, and inheritance of cytosine methylation in tomato nuclear DNA. Plant Mol. Biol. 16:753–770.
Michaelson, M.J., H.J. Price, J.R. Ellison and J.S. Johnston. 1991. Comparison of plant DNA contents determined by Feulgen microspectrophotometry and laser flow cytometry. Amer. J. Bot. 78:183–188.
An erratum to this article is available at http://dx.doi.org/10.1007/BF02672016.
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Arumuganathan, K., Earle, E.D. Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9, 208–218 (1991). https://doi.org/10.1007/BF02672069
- Genome size
- flow cytometry
- propidium iodide