Anti-human lung giant cell cancer (PG) effect of human LAK cellsin vitro and in nude mice

Abstract

Human LAK cells were prepared by culturing normal human peripheral blood mononuclear cells (PBMC) with or without rIL-2 and assayed for T cell surface markers as well as anti-tumor activity against PGin vitro and in nude mice. Although the percentages of T₃, T₄ and T₈ positive cells in rIL-2-activated cells did not differ significantly from those of control cellsin vitro, the former showed stronger cytotoxicity than control cells to PG tumor cellsin vitro. In vivo, LAK cells completely inhibited the growth of PG tumor in nude mice, whereas PBMC control cells were to be of no effect. The anti-tumor effect of human LAK cells in nude mice may offer a useful model to study the role of human LAK cells against human tumorin vivo.