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In vitro antitumor activity of tumor-infiltrating lymphocytes from human gastric carcinoma

  • Basic Investigations
  • Published:
Chinese Journal of Cancer Research

Abstract

Tumor-infiltrating lymphocytes (TIL) isolated from 11 gastric carcinoma were studied. TIL could grow for a long-term in medium containing recombinant interleukin-2(rIL-2). The mean expansion fold achieved in 6 long-term cultures of 11 specimens was 15.1 RIL-2 expanded gastric TIL exhibited significant cytotoxicity against K562, BGC823, MCF-7 and more effective antitumor cytotoxicity against fresh autologous tumor targets and human gastric cancer cell line. Peak cytotoxicity was shown in the third or fourth week after cultures. Cryopreservation of gastric TIL didn’t influence their expansion capacity and antitumor activity. Phenotypic analysis was demonstrated in this study. The results of present study indicate that TIL from human gastric carcinoma could be expanded and reach high levels of antitumor effector function in long-term cultures with rIL-2. Their function may be of clinical importance.

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Authors and Affiliations

  1. Beijing Institute for Cancer Research, Beijing, China

    Wang Hongzhi, Huang Xinfu, Lin Benyao, Yang Langui & Yao Lihua

Authors
  1. Wang Hongzhi
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  2. Huang Xinfu
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  3. Lin Benyao
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  4. Yang Langui
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  5. Yao Lihua
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Hongzhi, W., Xinfu, H., Benyao, L. et al. In vitro antitumor activity of tumor-infiltrating lymphocytes from human gastric carcinoma. Chinese Journal of Cancer Research 3, 18–21 (1991). https://doi.org/10.1007/BF02671285

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  • DOI: https://doi.org/10.1007/BF02671285

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