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Programmed multiple development: High performance thin layer chromatography

  • Symposium: Newest Analytical Methods
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

Poor resolution and sensitivity are not inherent in thin layer chromatography (TLC). They merely re-flect the fact that TLC, as normally practiced, is not a high performance (efficient) technique. When efforts are made to improve the efficiency of TLC, the re-sulting improvements in resolution and sensitivity make high performance TLC fully comparable to high performance liquid chromatography (HPLC). Two approaches to improved TLC performance are the use of small-particle TLC plates and the technique of Programmed Multiple Development (PMD). Prelimi-nary PMD methods for limonin in citrus juice and for caffeine in cola beverages clearly illustrate the use of PMD.

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Authors and Affiliations

  1. Regis Chemical Company, 8210 N. Austin Avenue, 60053, Morton Grove, Illinois

    T. H. Jupille

Authors
  1. T. H. Jupille
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Jupille, T.H. Programmed multiple development: High performance thin layer chromatography. J Am Oil Chem Soc 54, 179–182 (1977). https://doi.org/10.1007/BF02670792

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  • DOI: https://doi.org/10.1007/BF02670792

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