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Reduction of nuclear DNA contamination in the analysis of chloroplast DNA with restriction endonucleases

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Abstract

If chloroplasts purified on sucrose step gradients are treated for 10 min at 4°C with 2 M NaCl, followed by a 1000-g centrifugation, nuclear DNA contamination is reduced 1.5 to 3 fold as estimated by densitometry.

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Authors and Affiliations

  1. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia

    Jian-Wei Liu & Ray J. Rose

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  1. Jian-Wei Liu
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  2. Ray J. Rose
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Liu, JW., Rose, R.J. Reduction of nuclear DNA contamination in the analysis of chloroplast DNA with restriction endonucleases. Plant Mol Biol Rep 11, 48–53 (1993). https://doi.org/10.1007/BF02670559

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  • DOI: https://doi.org/10.1007/BF02670559

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