Skip to main content
SpringerLink
Log in

A method for extraction of total RNA fromPinus radiata and other conifers

  • Protocol
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Carbohydrates, polyphenolic compounds, terpenoids and tannins interfere with the extraction of intact, uncontaminated total RNA from conifers. A method for extraction of total RNA fromPinus radiata is described. This method uses cesium trifluoroacetate in the ultracentrifugal separation of RNA to overcome the problems of co-purification of contaminating secondary metabolites.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CsFTA:

cesium trifluoroacetate

References

  • Chomczinsky, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159.

    Article  Google Scholar 

  • Katterman, F.R.H. and V.I. Shattuck. 1983. An effective method of DNA isolation from mature leaves ofGossypium species that contain large amounts of phenolic terpenoids and tannins. Prep. Biochem. 13:347–359.

    Article  PubMed  CAS  Google Scholar 

  • Manning, K. 1991. Isolation of nucleic acids from plants by differential solvent precipitation. Anal. Biochem. 195:45–50.

    Article  PubMed  CAS  Google Scholar 

  • Neal, D.B. and C.G. Williams 1991. Restriction fragment length polymorphism mapping in conifers and applications to forest genetics and tree improvement. Can. J. Forest Res. 21:545–554.

    Google Scholar 

  • Okayama, H., M. Kawaichi, M. Brownstein, F. Lee, T. Yokota and K. Arai. 1987. High-efficient cloning of full-length cDNA; Construction and screening of cDNA expression libraries for mammalian cells. Meth. Enzymol. 154:3–41.

    Article  PubMed  CAS  Google Scholar 

  • Reddy, A.S.N., P.K. Jena, S.K. Mukherjee and B.W. Poovaiah. 1990. Molecular cloning of cDNA for auxin-induced mRNAs and developmental expression of the auxin-inducible genes. Plant Mol. Biol. 14:643–653.

    Article  PubMed  CAS  Google Scholar 

  • Sanchez, F., F. Campos, J. Padilla, J. Bonneville, C. Enriquez and D. Caput. 1987. Purification, cDNA cloning and developmental expression of the nodule-specific uricase fromPhaseolus vulgaris L. Plant Physiol. 85:1143–1147.

    Article  Google Scholar 

  • Verwoerd, T.C., B.M.M. Dekker and A. Hoekema. 1989. A small-scale procedure for the rapid isolation of plant RNAs. Nucl. Acids. Res. 6:5415.

    Google Scholar 

  • Yeh, K., R. Juang and J. Su. 1991. A rapid and efficient method, for RNA isolation from plant with high carbohydrate content. Focus 13:102–103.

    Google Scholar 

  • Zobel, B.J. 1977. Review of the Contribution on Wood Quality. pp. 143–145. In:Progress and Problem of Genetic Improvement of Tropical Trees. (eds. D.G. Nickles, J. Burley and R.D. Barnes) Oxford, UK, Commonwealth Forestry Institute.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Queensland Agricultural Biotechnology Centre, Department of Primary Industries, Gehremann Laboratories, Research Rd., The University of Queensland, 4072, St. Lucia, QLD, Australia

    Glenn C. Graham

Authors
  1. Glenn C. Graham
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Graham, G.C. A method for extraction of total RNA fromPinus radiata and other conifers. Plant Mol Biol Rep 11, 32–37 (1993). https://doi.org/10.1007/BF02670557

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02670557

Key Words

Search

Navigation