Plant Molecular Biology Reporter

, Volume 11, Issue 2, pp 113–116 | Cite as

A simple and efficient method for isolating RNA from pine trees

  • Shujun Chang
  • Jeff Puryear
  • John Cairney
Commentary

Key words

phenolic compounds pine Pinus polysaccharides RNA extraction RNase 

Abbreviations

CTAB

hexadecyltrimethylammonium bromide

IAA

isoamyl alcohol

SSTE

defined inMaterials and Methods

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baker, S. S., C. L. Rugh, and J. C. Kamalay. 1990. RNA and DNA isolation from recalcitrant plant tissues. Biotechniques 9(3):268–272.PubMedGoogle Scholar
  2. Fang, G., S. Hammar, and R. Grumet. 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13:52–55.PubMedGoogle Scholar
  3. Katterman, F.R.H., and V.I. Shattuck. 1983. An effective method of DNA isolation from the mature leaves ofGossypium species that contain large amounts of phenolic terpenoids and tannins. Prep. Biochem. 13:347–359.PubMedCrossRefGoogle Scholar
  4. Sambrook, J., E. F. Fritsch, and T. Maniatis, 1989.Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, New York.Google Scholar
  5. Schneiderbauer, A., H. Sandermann Jr., and D. Ernst. 1991. Isolation of functional RNA from plant tissues rich in phenolic compounds. Anal. Biochemistry 197:91–95.CrossRefGoogle Scholar

Copyright information

© International Society for Plant Molecular Biology 1993

Authors and Affiliations

  • Shujun Chang
    • 1
  • Jeff Puryear
    • 1
  • John Cairney
    • 1
  1. 1.Department of Forest ScienceTexas A&M UniversityCollege StationUSA

Personalised recommendations