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Polymorphisms in grapevine DNA detected by the RAPD PCR technique

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Abstract

A sensitivie, reproducible technique is described for detecting polymorphisms between the nuclear DNA of grapevine isolates using the RAPD PCR technique. Unique fingerprints of a number of cultivars were readily distinguished by using either single primers or mixtures of two primers. The method will be used to provide a databank of fingerprints for the rapid identification of grapevine cultivars, and to develop phylogenetic relationships. It will also be extended and modified in an attempt to detect polymorphisms between DNAs of clonal selections of individual cultivars.

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Abbreviations

AP-PCR:

arbitrarily primed polymerase chain reaction

gp32:

gene 32 protein from T4

RAPD:

random amplified polymorphic DNA

TBE:

trisborate-EDTA buffer

References

  • Bachmann, O., and R. Blaich. 1988. Isoelectric focusing of grapevine peroxidases as a tool for ampellography. Vitis 27:147–155.

    CAS  Google Scholar 

  • Bassam, B.J., G. Caetano-Anollés, and P.M. Gresshof. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196:80–83.

    Article  PubMed  CAS  Google Scholar 

  • Bourquin, J.-C., P. Tournier, L. Otten, and B. Walter. 1992. Identification of sixteen grapevine rootstocks by RFLP and RFLP analysis of nuclear DNA extracted from the wood. Vitis 31:157–162.

    CAS  Google Scholar 

  • Caetano-Anollés, G., B.J. Bassam, and P.M. Gresshoff. 1992. Primer-template interactions during DNA amplification fingerprinting with single arbitrary oligonucleotides. Mol. Gen. Genet. 235:157–165.

    Article  PubMed  Google Scholar 

  • Collins, G.G., and R.H. Symons. 1992. Extraction of nuclear DNA from grapevine leaves by a modified procedure. Plant Mol. Biol. Reptr. 10:233–235.

    Article  CAS  Google Scholar 

  • Debener, T., F. Salamini, and C. Gebhardt. 1990. Phylogeny of wild and cultivatedSolanum species based on nuclear restriction fragment length polymorphisms (RFLPs). Theor. Appl. Genet. 79:360–368.

    Article  CAS  Google Scholar 

  • Hu, J., and C.F. Quiros. 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Reports 10:505–511.

    Article  Google Scholar 

  • Klein-Lankhorst, R.M., A. Vermunt, R. Weide, T. Liharska, and P. Zabel. 1991. Isolation of molecular markers from tomato (L. esculentum) using random amplified polymorphic DNA (RAPD). Theor. Appl. Genet. 83:108–114.

    Article  CAS  Google Scholar 

  • Panaccio, M., and A. Lew. 1991. PCR based diagnosis in the presence of 8% (v/v) blood. Nucleic Acids Res. 19:1151.

    Article  PubMed  CAS  Google Scholar 

  • Quiros, C.F., J. Hu, P. This, A.M. Chevre, and M. Delseny. 1991. Development and chromosomal localization of genome-specific markers by polymerase chain reaction inBrassica. Theor. Appl. Genet. 82:627–632.

    Article  CAS  Google Scholar 

  • Schwarz, K., T. Hansen-Hagge, and C. Bartram. 1990. Improved yields of long PCR products using gene 32 protein. Nucleic Acids Res. 18:1079.

    Article  PubMed  CAS  Google Scholar 

  • Szychowski, J.A., A.C. Goheen, and J.S. Semancik. 1988. Mechanical transmission and rootstock reservoirs as factors in the widespread distribution of viroids in grapevines. Am. J. Enol. Vitic. 39:213–216.

    Google Scholar 

  • Tzuri, G., J. Hillel, U. Lavi, A. Haberfield, and A. Vainstein. 1991. DNA fingerprint analysis of ornamental plants. Plant Science 76:91–97.

    Article  CAS  Google Scholar 

  • Weeden, N.F., B.I. Reisch, and M.E. Martens. 1988. Genetic analysis of isozyme polymorphism in grape. J. Amer. Soc. Hort. Sci. 113:765–769.

    CAS  Google Scholar 

  • Weining, S., and P. Langridge. 1991. Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theor. Appl. Genet. 82:209–216.

    Article  CAS  Google Scholar 

  • Welsh, J., R.J. Honeycutt, M. McClelland, and B.W.S. Sobral. 1991. Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theor. Appl. Genet. 82:473–476.

    Article  CAS  Google Scholar 

  • Wolfe, W.H.. 1976. Identification of grape varieties by isozyme banding patterns. Am. J. Enol. Vitic. 27:68–73.

    CAS  Google Scholar 

  • Yamamoto, N., G. Ono, K. Takashima, and A. Totsuka. 1991. Restriction fragment length polymorphisms of grapevine DNA with phenylalanine ammonia-lyase cDNA. Japan. J. Breed. 41:365–368.

    CAS  Google Scholar 

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Collins, G.G., Symons, R.H. Polymorphisms in grapevine DNA detected by the RAPD PCR technique. Plant Mol Biol Rep 11, 105–112 (1993). https://doi.org/10.1007/BF02670467

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