Abstract
Chlorophyll pigments in crude and degummed canola oils were analyzed by spectrophotometry using a modified AOCS Method and by reversed phase HPLC. HPLC showed that crude canola oils contained very littlechlorophyll a orb, these pigments having been converted to pheophytins and other pigments with similar spectral properties. The ratio ofchlorophyll a∶b in the seed was found to be about 3∶1 while the ratio ofpheophytin a∶b in the oil was about 9∶1. As the AOCS Method for determining oil chlorophyll was calibrated for pure chlorophyll, the use of this method on crude canola oil results in a significant error. Recalibration of the spectrophotometric procedure with pheophytin gave better agreement with the HPLC method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dahlen, J.A.H., J. Amer. Oil Chem. Soc.50:312A (1973).
Daun, J.K., J. Amer. Oil Chem. Soc.59:15 (1982).
Pritchard, J.R., J. Amer. Oil Chem. Soc.60:322 (1983).
Usuki, R., Y. Endo, T. Suzuki, and T. Kaneda, Fat Science, Proc. 16th ISF Congress, Budapest, 1983. J. Hollo (ed.) Elsevier, Amsterdam, 1985, p. 627.
Endo, Y., R. Usuki, and T. Kaneda, Agric. Biol. Chem.48:985 (1984).
Endo, Y., R. Usuki, and T. Kaneda, J. Amer. Oil Chem. Soc.61: 781 (1984).
Fakourelis N., E.C. Lee, and D.B. Min, J. Food Sci.52:234 (1987).
Endo, Y., R. Usuki, and T. Kaneda, J. Amer. Oil Chem. Soc.62:1375 (1985).
Endo, Y., R. Usuki, and T. Kaneda, J. Amer. Oil Chem. Soc.62:1385 (1985).
Abraham, Y., and J. DeMan, J. Amer. Oil Chem. Soc.63:1185 (1986).
Mag, T.K., J. Amer. Oil Chem. Soc.60:332A (1982).
Official and Tentative Methods of the American Oil Chemists’ Society, 3rd edn., Edited by V.C. Mehlenbacher, T.H. Hopper, E.M. Smallee, W.E. Link, R.O. Walker, and R.C. Walker, American Oil Chemists’ Society, Champaign, Method Cc 13d-55.
Stillman, R.C., J. Amer. Oil Chem. Soc.30:159 (1953).
Stillman, R.C., J. Amer. Oil Chem. Soc.31:469 (1954).
Stillman, R.C., J. Amer. Oil Chem. Soc.32:503 (1955).
Yuen, W., and P. Kelly, in Analytical Chemistry of Rapessed and Its Products—A Symposium, J.K. Daun, D.I. McGregor and E.E. McGregor (eds.), Canola Council of Canada, Winninpeg, (1980), p. 139.
National Standard of Canada, Canola Oil, Crude, Degummed and Refined. Can/CGSB-32.300-M87. Canadian General Standards Board, Ottawa, (1987).
Niewiadomski, H., Bratkowska, I., and E. Mossakowska, J. Amer. Oil Chem. Soc.42:731 (1965).
Box, J.A.G., and H.A. Boekenoogen., Feete, Seifen, Anstrichmettel69:724 (1967).
R. Usuki, T. Suzuki, Y. Endo, and T. Kaneda, J. Amer. Oil Chem. Soc.61:785 (1984).
Suzuki, T., Nishioka, A., and R. Usuki, Yukagaku (J. Japan. Oil Chem. Soc.)34:933 (1985).
Davidek, J., J. Pokorny, and J. Velisek, Prumysl Potravin37:351 (1986).
Fraser, M.S., and G. Frankl, J. Amer. Oil Chem. Soc.62:113 (1985).
Rahmani, M., and A.S. Csallany., Rev. Franc. des Corps Gras32:257 (1985).
Vernon, L.P., Analytical Chem.32:1114 (1960).
Association of Official Analytical Chemists' Official Methods of Analysis., edited by W. Horowitz, A.O.A.C., Washington, 1980, Method 3. 132.
Goedheer, J.C. in The Chlorophylls edited by L.P. Vernon and G.R. Seely, Academic Press, New York, 1966, p. 147.
Johansson, S.A., and L.-A. Appelqvist., Fette Seifen Anstrichmittel86:304 (1984).
Khachik, F., G.R. Beecher, and N.F. Whittaker, Agric. Food. Chem.34:603 (1986).
Khachik, F., and G.R. Beecher, J. Agric. Food Chem.35:732 (1987).
Hurley, J.P., J. Analysis Purific.. (June):12 (1988).
Usuki, R., and Y. Endo, J. Japan Oil Chem. Soc.36:21 (1987).
Author information
Authors and Affiliations
About this article
Cite this article
Daun, J.K., Thorsteinson, C.T. Determination of chlorophyll pigments in crude and degummed canola oils by HPLC and spectrophotometry. J Am Oil Chem Soc 66, 1124–1128 (1989). https://doi.org/10.1007/BF02670098
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02670098