Abstract
We describe a modified protocol to perform a tissue print northern using digoxigenin-labeled riboprobes. The result of the hybridization is directly visualized on the membrane by an alkaline phosphatase detection system.
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Cassab, G.I. and J.E. Varner. 1987. Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper. J. Cell Biol. 105:2581–2588.
Ye, Z.-H., Y.-R. Song, and J.E. Varner.1992. Gene expression in plants. In:Tissue printing, Tools for the Study of Anatomy, Histochemistry and Gene Expression. (eds. P.D. Reid and R.F. Pont-Lezica), pp. 95–123. Academic Press, San Diego.
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Tiré, C., Van Montagu, M. & Engler, G. A new method to perform nonradioactive tissue printing using digoxigenin-labeled riboprobes. Plant Mol Biol Rep 11, 216–219 (1993). https://doi.org/10.1007/BF02669848
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DOI: https://doi.org/10.1007/BF02669848