Abstract
Fresh or frozen tissue is usually used as a source of DNA for PCR and RAPD analysis. We have found that leaves can be allowed to dry at room temperature before extraction of DNA. Heating the leaves or microwave drying resulted in poor recovery of DNA. Storage of fresh leaves in paper envelopes in the laboratory was the most successful approach. This allowed the tissue to dry out over a period of several days and DNA could be extracted at any time, providing a convenient method for the collection and analysis of field material. DNA from leaves stored for four months at room temperature was suitable for PCR analysis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- RAPD:
-
random amplified polymorphic DNA
References
Bult, C., M. Kallersjo, and Y. Suh. 1992. Amplification and sequencing of 16/18S rDNA from gel-purified total plant DNA. Plant Mol. Biol. Reptr. 10:273–284.
Graham, G.C., P. Mayers, and R.J. Henry 1993. A simple and rapid method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques (in press).
Kolchinsky, A., M. Kolchinsky, and E. Ananiev. 1991. ‘Portraying’ of plant genomes using the polymerase chain reaction amplification of ribosomal 5S genes. Genome 34:1028–1031.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski, and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18:6531–6535.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thomson, D., Henry, R. Use of DNA from dry leaves for PCR and RAPD analysis. Plant Mol Biol Rep 11, 202–206 (1993). https://doi.org/10.1007/BF02669845
Issue Date:
DOI: https://doi.org/10.1007/BF02669845