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A clearing technique for histochemical location of GUS activity in pollen tubes and ovules ofLycopersicon

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Abstract

We describe a protocol to locate GUS activity, remove browning due to polyphenolics and clear floral tissue fromLycopersicon species. Suggestions are given to adapt this protocol for samples from other species.

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Abbreviations

GUS:

β-glucuronidase

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Authors and Affiliations

  1. Department of Plant Breeding, Cornell University, 252 Emerson, 14853-1901, Ithaca, NY

    B. E. Liedl & M. A. Mutschler

  2. Plant Gene Expression Center, USDA/ARS, UC-Berkeley, 800 Buchanan St., 94710, Albany, CA, USA

    S. McCormick

Authors
  1. B. E. Liedl
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  2. S. McCormick
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  3. M. A. Mutschler
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Liedl, B.E., McCormick, S. & Mutschler, M.A. A clearing technique for histochemical location of GUS activity in pollen tubes and ovules ofLycopersicon . Plant Mol Biol Rep 11, 194–201 (1993). https://doi.org/10.1007/BF02669844

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