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Purification of chloroplast DNA using hexadecyltrimethylammonium bromide

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Abstract

A simple and efficient method of extracting purified chloroplast DNA is described. Chloroplasts are isolated in a high-salt buffer, and lysed with hexadecyltrimethylammonium bromide. The DNA is extracted with chloroform and precipitated. This rapid procedure has been successful for a taxonomically diverse group of plants, and eliminates many of the time-consuming or expensive steps involved in other protocols. As a result, it may be a useful procedure to try prior to resorting to more complex alternatives.

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Abbreviations

CTAB:

hexadechyltrimethyla molnium bromide

EDTA:

ethylenediamine tetraacetate

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Authors and Affiliations

  1. Department of Botany, University of Texas at Austin, 78713-7640, Austin, Texas, U.S.A.

    Brook G. Milligan

  2. Department of Biology, University of Michigan, 48109, Ann Arbor, Michigan, U.S.A.

    Brook G. Milligan

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  1. Brook G. Milligan
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Milligan, B.G. Purification of chloroplast DNA using hexadecyltrimethylammonium bromide. Plant Mol Biol Rep 7, 144–149 (1989). https://doi.org/10.1007/BF02669630

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  • DOI: https://doi.org/10.1007/BF02669630

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