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Chloroplast DNA isolation from higher plants: An improved non-aqueous method

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Abbreviations

cpDNA:

chloroplast DNA

RFLP:

restriction fragment length polymorphism

NET:

100 mM NaCl, 30 mM EDTA, pH 8.0, 80 mM Tris-HCl

TE:

10 mM Tris, 1 mM EDTA, pH 8

TX:

Triton X-100

References

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Authors and Affiliations

  1. ORSTOM/CNRS, Centre Emberger, B.P. 5051, 34033, Montpellier Cedex, France

    Andreas M. Dally & Gérard Second

  2. FB Biologie, Abt. Cytologie, Universität Kaiserslautern, Erwin-Schrödinger-Str., D-6750, Kaiserslautern, FRG

    Andreas M. Dally

  3. Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, USA

    Gérard Second

Authors
  1. Andreas M. Dally
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  2. Gérard Second
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Dally, A.M., Second, G. Chloroplast DNA isolation from higher plants: An improved non-aqueous method. Plant Mol Biol Rep 7, 135–143 (1989). https://doi.org/10.1007/BF02669629

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  • DOI: https://doi.org/10.1007/BF02669629

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