Abstract
A new two-dimensional gel technique has been developed that makes it possible to load large amounts of protein and still retain good resolution. Protein samples are added to the isoelectric-focusing acrylamide solution prior to polymerization. Focusing times are short, making it possible to complete both dimensions of a two-dimensional gel in five to six hours. The technique yields larger amounts of separated protein for sequencing, and also greatly increases the quality of two-dimensional immunoblots.
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Abbreviations
- 2D:
-
two-dimensional
- ddH2O:
-
double-distilled water
- IEF:
-
isoelectric focusing
References
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Barent, R.L., Elthon, T.E. Two-dimensional gels: An easy method for large quantities of proteins. Plant Mol Biol Rep 10, 338–344 (1992). https://doi.org/10.1007/BF02668910
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DOI: https://doi.org/10.1007/BF02668910