Skip to main content
Log in

Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana

  • Arabidupdate
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

BAC:

bacterial artificial chromosome

IPTG:

isopropylthio-β-D-galactoside

LB:

Luria-Bertani medium

YAC:

yeast artificial chromosome

X-Gal:

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside

References

  • Gibson, S.I. and C. Somerville, 1992. Chromsome walking inArabidopsis thaliana using yeast artificial chromosomes. In:Methods in Arabidopsis Research. (eds. Koncz, C.N.-H. Chua, J. Schell) World Scientific, Singapore. pp 119–143.

    Google Scholar 

  • Sambrook, J., E.F. Fritsch, T. Maniatis. 1989.Molecular Cloning. A Laboratory Manual. 2nd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    Google Scholar 

  • Shizuya, H. B. Birren, U.-J. Kim, V. Mancino, T. Slepak, Y. Tachiiri and M. Simon. 1992. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA inEscherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. 89:8794–8797.

    Article  PubMed  CAS  Google Scholar 

  • Woo, S.-S., J. Jiang, B.S. Gill, A.H. Paterson, R.A. Wing. 1994. Construction and characterization of a bacterial artificial chromosome library ofSorghum bicolor. Nucleic Acid Res. 22:4922–4931.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, H.-B., Z. Zhao, X. Ding, A. H. Paterson and R.A. Wing. 1995. Preparation of megabase-size DNA from plant nuclei. Plant J 7:175–184

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Rod A. Wing.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, S., Creelman, R.A., Mullet, J.E. et al. Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana . Plant Mol Biol Rep 13, 124–128 (1995). https://doi.org/10.1007/BF02668782

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02668782

Keywords

Navigation