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Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana

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We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

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Correspondence to Rod A. Wing.

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Choi, S., Creelman, R.A., Mullet, J.E. et al. Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana . Plant Mol Biol Rep 13, 124–128 (1995).

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