Abstract
We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.
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Abbreviations
- BAC:
-
bacterial artificial chromosome
- IPTG:
-
isopropylthio-β-D-galactoside
- LB:
-
Luria-Bertani medium
- YAC:
-
yeast artificial chromosome
- X-Gal:
-
5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside
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Choi, S., Creelman, R.A., Mullet, J.E. et al. Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana . Plant Mol Biol Rep 13, 124–128 (1995). https://doi.org/10.1007/BF02668782
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DOI: https://doi.org/10.1007/BF02668782