Abstract
Extraction of RNA from alfalfa pollen using conventional grinding devices resulted in low yields of degraded RNA; degradation appeared to be related to the length of time required to break open the pollen grains with such devices. A glass syringe was modified to provide the restricted, shearing environment necessary for rapidly rupturing this type of tissue. This apparatus would be useful for extracting a variety of molecules from pollen of any species, but is particularly valuable for species which produce low amounts of relatively small pollen grains.
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Abbreviations
- ATA:
-
aurintricarboxylic acid
- DTT:
-
dithiothreitol
- DEPC:
-
diethylpyrocarbonate
- PVP:
-
polyvinylpyrrolidone
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Qiu, X., Erickson, L. A simple and effective method for isolating RNA from alfalfa pollen. Plant Mol Biol Rep 12, 209–214 (1994). https://doi.org/10.1007/BF02668743
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DOI: https://doi.org/10.1007/BF02668743