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Isolation of RNA from floral tissue ofRumex acetosa (Sorrel)

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Abstract

Flower tissue ofRumex acetosa was previously intractable for the isolation of RNA using standard methods, due probably to its high level of polysaccharides. Extraction at low pH, precipitation of polysaccharides with potassium acetate followed by precipitation of RNA with lithium chloride yielded high-quality RNA that was suitable for northern hybridisation,in-vitro translation, poly(A)+ RNA selection, and subsequent cDNA synthesis.

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Abbreviations

DEPC:

diethylpyrocarbonate

DTT:

dithiothreitol

References

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Ainsworth, C. Isolation of RNA from floral tissue ofRumex acetosa (Sorrel). Plant Mol Biol Rep 12, 198–203 (1994). https://doi.org/10.1007/BF02668741

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  • DOI: https://doi.org/10.1007/BF02668741

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