Abstract
Flower tissue ofRumex acetosa was previously intractable for the isolation of RNA using standard methods, due probably to its high level of polysaccharides. Extraction at low pH, precipitation of polysaccharides with potassium acetate followed by precipitation of RNA with lithium chloride yielded high-quality RNA that was suitable for northern hybridisation,in-vitro translation, poly(A)+ RNA selection, and subsequent cDNA synthesis.
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Abbreviations
- DEPC:
-
diethylpyrocarbonate
- DTT:
-
dithiothreitol
References
Ainsworth, C.C., Clark, J. and J. Balsdon. 1993. Expression, organisation and structure of the genes encoding thewaxy protein (granule bound starch synthase) in wheat. Plant Mol. Biol. 22:67–82.
Bahloul, M. and G. Burkard. 1993. An improved method for the isolation of total RNA from spruce tissues. Plant Mol. Biol. Reptr 11:212–215.
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Ainsworth, C. Isolation of RNA from floral tissue ofRumex acetosa (Sorrel). Plant Mol Biol Rep 12, 198–203 (1994). https://doi.org/10.1007/BF02668741
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DOI: https://doi.org/10.1007/BF02668741