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A non-destructive assay for GUS in the media of plant tissue cultures

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Abstract

β-glucuronidase (GUS) can be assayed in the spent media of plant tissues transformed with some GUS gene fusions (Jefferson, 1988). This approach is based on the presence of GUS in the media of transformed plant tissues expressing the gene and can be used to monitor the progress of transformation without destruction of the tissue under study.

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Abbreviations

uid A :

structural gene coding for β-glucuronidase

lacZ :

structural gene coding for β-galactosidease

GUS:

β-glucuronidase

MUG:

4-methylumbelliferyl glucuronide

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Authors and Affiliations

  1. Department of Soil and Crop Sciences, Texas A & M University, 77843, Texas, College Station, USA

    Jean H. Gould & Roberta H. Smith

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  1. Jean H. Gould
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  2. Roberta H. Smith
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Gould, J.H., Smith, R.H. A non-destructive assay for GUS in the media of plant tissue cultures. Plant Mol Biol Rep 7, 209–216 (1989). https://doi.org/10.1007/BF02668687

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  • DOI: https://doi.org/10.1007/BF02668687

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