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An easy technique for the clearing of histochemically stained plant tissue

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Abstract

The sites of GUS-activity in strongly pigmented whole-mount preparations or thick handmade sections of plant material can easily be visualized by clearing it with chlorallactophenol (CLP), without affecting the ClBr-indigo precipitate.

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Abbreviations

GUS:

β-glucuronidase

X-gluc:

5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid

CLP:

chlorallactophenol

CIBr-indigo:

dichloro-dibromoindigo

References

  • Amann, J. 1899. Neue Beobachtungsmedien. Zeitschr. wiss. Mikr. 16:38.

    Google Scholar 

  • Bersier, J.-D. and G. Bocquet. 1960 Les méthodes d'éclaircissement en vascularisation et en morphogénie végétales comparées. Arch. Sci. 13:555–566

    Google Scholar 

  • Craig, S. 1992. The GUS reporter gene. Application to light and transmission electron microscopy. In:GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression, pp. 115–124, S.R. Gallagher, ed. Academic Press; Inc. San Diego.

    Google Scholar 

  • Gallagher, S.R. (ed.) 1992.Gus Protocols: Using the Gus Gene as a Reporter of Gene Expression. Academic Press, Inc. San Diego.

    Google Scholar 

  • Herr, J.M., Jr. 1971. A new clearing-squash technique for the study of ovule development in Angiosperms. Amer. J. Bot. 58:785–790.

    Article  Google Scholar 

  • Jefferson, R.A., S.M. Burgess and D. Hirsh. 1986. β-Glucuronidase fromEscherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. USA. 83:8447–8451.

    Article  PubMed  CAS  Google Scholar 

  • Jefferson, R.A., T.A. Kavanagh and M.W. Bevan. 1987. GUS fusions: β-Glucuronidase as a sensitive and versatile gene marker in higher plants. EMBO J. 6:3901–3907.

    PubMed  CAS  Google Scholar 

  • Van Cotthem, W. and E. Fryns-Claessens. 1972.Plantenanatomie in Praktijk. J. Van In, Lier.

    Google Scholar 

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Authors and Affiliations

  1. Laboratorium voor morfologie, systematiek en ecologie van de planten, Universiteit Gent, B-9000, Gent, Belgium

    Tom Beeckman

  2. Laboratoire Associé de l’Institut National de la Recherche Agronomique (France), Universiteit Gent, B-9000, Gent, Belgium

    Gilbert Engler

Authors
  1. Tom Beeckman
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  2. Gilbert Engler
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Beeckman, T., Engler, G. An easy technique for the clearing of histochemically stained plant tissue. Plant Mol Biol Rep 12, 37–42 (1994). https://doi.org/10.1007/BF02668662

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  • DOI: https://doi.org/10.1007/BF02668662

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